High expression of FER tyrosine kinase predicts poor prognosis in clear cell renal cell carcinoma.
High expression of FER tyrosine kinase predicts poor prognosis in clear cell renal cell carcinoma.
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FER酪氨酸激酶高表达预示透明细胞肾细胞癌预后不良
DOI：
10.3892/ol.2012.1032
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发表时间：
2013-02
期刊：
影响因子：
2.9
通讯作者：
Ye J
中科院分区：
文献类型：
--
作者：
Wei C;Wu S;Li X;Wang Y;Ren R;Lai Y;Ye J
FER酪氨酸激酶（FER）已被证明在肿瘤发生和转移中起关键作用;然而，其作为透明细胞肾细胞癌（ccRCC）的新型预后标志物的潜在价值仍不清楚。在48例配对的ccRCC和正常邻近组织（ADT）中，使用实时PCR来评估FER mRNA的表达。采用免疫组织化学方法检测87例ADT和206例ccRCC中FER蛋白的表达。统计学分析用于检查FER表达水平与ccRCC患者临床特征之间的相关性。在ccRCC组织和ADT之间确定了FER mRNA水平的显著差异。免疫组织化学分析显示，87例ccRCC样本中FER蛋白的表达高于配对ADT。此外，206例ccRCC中FER蛋白表达与肿瘤大小、T分期、N分类、转移、复发和Fuhrman分级显著相关，而与年龄和性别的相关性未被确定。Kaplan-Meier生存分析显示，与FER水平较低的患者相比，FER水平较高的患者的生存结局较差。经对数秩检验，两组累积生存率差异有统计学意义。考克斯回归分析显示，FER表达、N分期和远处转移是影响ccRCC患者总生存期的独立预后因素。我们的研究结果表明，肿瘤组织中FER的过度表达预示着ccRCC患者的预后不良，FER可能成为ccRCC的一种新的预后标志物。
FER tyrosine kinase (FER) has been demonstrated to play a critical role in tumorigenesis and metastasis; however, its potential value as a novel prognostic marker for clear cell renal cell carcinoma (ccRCC) remains unclear. In 48 paired samples of ccRCCs and normal adjacent tissues (ADTs), real-time PCR was used to evaluate the expression of FER mRNA. The expression of FER protein was assessed in 87 ADTs and 206 samples of ccRCC using immunohistochemical methods. Statistical analysis was used to examine the correlations between the expression levels of FER and the clinical characteristics of ccRCC patients. A significant difference was identified between ccRCC tissues and ADTs in the mRNA levels of FER. Immunohistochemistry analyses revealed higher expression of FER protein in 87 ccRCC samples compared to the paired ADTs. In addition, FER protein expression in 206 ccRCC samples was significantly correlated with tumor size, T stage, N classification, metastasis, recurrence and Fuhrman grade, while associations with age and gender were not identifed. The Kaplan-Meier survival analysis showed that patients with high FER levels had a poorer survival outcome compared with those with lower levels. The log-rank test demonstrated that the cumulative survival rates were significantly different between the two groups. The Cox regression analysis indicated that FER expression, N stage and distant metastasis were independent prognostic factors for overall survival of ccRCC patients. Our results indicate that overexpression of FER in tumor tissues predicts a poor prognosis of patients with ccRCC, and FER may serve as a novel prognostic marker for ccRCC.
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