The expression dynamics of IL-17 and Th17 response relative cytokines in the trachea and spleen of chickens after infection with Cryptosporidium baileyi.
The expression dynamics of IL-17 and Th17 response relative cytokines in the trachea and spleen of chickens after infection with Cryptosporidium baileyi.
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贝氏隐孢子虫感染鸡气管和脾脏中IL-17和Th17反应相关细胞因子的表达动态
DOI：
10.1186/1756-3305-7-212
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发表时间：
2014-05-06
影响因子：
3.2
通讯作者：
Yu SK
中科院分区：
文献类型：
--
作者：
Zhao GH;Cheng WY;Wang W;Jia YQ;Fang YQ;Du SZ;Yu SK
背景贝氏隐孢子虫（Cryptosporidiumbaileyi）是禽类隐孢子虫的优势种，也是研究隐孢子虫生物学的模型。IL-17（又称IL-17 A）是Th 17细胞的标志性促炎细胞因子，在多种人类自身免疫性疾病和多种动物微生物感染性疾病中发挥重要作用，并可能在隐孢子虫感染中发挥作用。结果与未感染对照组相比，感染组鸡气管组织中细胞因子的表达均显著上调，与未感染对照组相比，感染组鸡气管组织中细胞因子的表达显著上调。贝氏感染。气管中IL-17 mRNA水平在感染后12 h即显著升高，在感染后24 h和10 d达到高峰，此后逐渐下降。脾脏IL-17和Th 17应答相关细胞因子的转录水平也在感染过程中的不同时间点显著升高。结论IL-17参与了某些胞内原虫感染过程中的炎症诱导。本研究结果提示IL-17可能在隐孢子虫感染的免疫应答中起作用，为进一步研究Th 17细胞在隐孢子虫感染中的作用提供了基础资料。
BackgroundCryptosporidium baileyi is the dominant Cryptosporidium species in birds causing emerging health problems in the poultry industry, and is also a model to study the biology of Cryptosporidium spp.. IL-17 (also called IL-17A) is a hallmark pro-inflammatory cytokine of Th17 cells that plays an important role in several human autoimmune diseases and microbial infection disease of many animals, and it may play a role in Cryptosporidium infection.MethodsThe present study examined the mRNA level of IL-17 and Th17 response relative cytokines in the trachea and spleen of C. baileyi-infected chickens at different time points using real-time quantitative PCR (qPCR).ResultsAll examined cytokines in the trachea were up-regulated in the infected chickens compared with the uninfected control during C. baileyi infection. Significant increased IL-17 mRNA level in the trachea was observed as early as 12 h post infection (pi), peaking at 24 h pi and 10 d pi, and declining thereafter. The transcription levels of IL-17 and Th17 response relative cytokines in spleen were also significantly increased at different time points during the infection.ConclusionsIL-17 was indicated to participate in the induction of inflammation during infection of some intracellular protozoan parasites. The results in the present study suggest that IL-17 may play a role in immunity against Cryptosporidium infection, and provide basic information for determining the role of Th17 cell in Cryptosporidium infection.
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影响因子：
4.9
作者：
Hwang SY;Kim JY;Kim KW;Park MK;Moon Y;Kim WU;Kim HY
通讯作者：
Kim HY
影响因子：
3.2
作者：
Koh W;Clode PL;Monis P;Thompson RC
通讯作者：
Thompson RC
影响因子：
3.1
作者：
Ferreira, Maria Carolina;Whibley, Natasha;Gaffen, Sarah L.
通讯作者：
Gaffen, Sarah L.
影响因子：
5.8
作者：
McNair, Nina N.;Mead, Jan R.
通讯作者：
Mead, Jan R.
DOI：
10.1111/j.1550-7408.1986.tb05608.x
发表时间：
1986-05-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
CURRENT, WL;UPTON, SJ;HAYNES, TB
通讯作者：
HAYNES, TB