Multiplication of the waterborne pathogen Cryptosporidium parvum in an aquatic biofilm system.
Multiplication of the waterborne pathogen Cryptosporidium parvum in an aquatic biofilm system.
复制标题
DOI：
10.1186/1756-3305-6-270
复制
发表时间：
2013-09-19
影响因子：
3.2
通讯作者：
Thompson RC
中科院分区：
文献类型：
--
作者：
Koh W;Clode PL;Monis P;Thompson RC
在自然水生环境中，生物膜被认为是小隐孢子虫卵囊的环境储存库。然而，这些卵囊在生物膜内的命运尚未确定。本研究旨在通过使用定量聚合酶链反应（qPCR）测量寄生虫数量随时间的变化并使用共聚焦显微镜和免疫标记技术检测可能的细胞外发育阶段来确定生物膜是否有能力支持隐孢子虫的繁殖。建立了铜绿假单胞菌生物膜流动细胞系统，并在六天的时间内持续供应微小假单胞菌卵囊。随着生物膜的成熟，检测到隐孢子虫显着增加（P<0.001），在此期间隐孢子虫总数增加了 2-3 倍。由此，从生物膜中鉴定出各种隐孢子虫发育阶段（子孢子、滋养体、I 型和 II 型半体）。这是第一项研究表明，生物膜不仅可以作为卵囊的环境储存库，而且还能够支持隐孢子虫在水生环境中随着时间的推移而繁殖。
In natural aquatic environments biofilms are known to act as environmental reservoirs for Cryptosporidium parvum oocysts. However, the fate of these oocysts within biofilms has yet to be determined. This study aimed to identify if biofilms have the ability to support the multiplication of Cryptosporidium by measuring the change in parasite number over time using quantitative polymerase chain reaction (qPCR) and detecting the possible extracellular developmental stages using a combination of confocal microscopy and immunolabelling techniques. Pseudomonas aeruginosa biofilm flow cell systems were established and C. parvum oocysts were constantly supplied over a six day period. A significant (P < 0.001) increase in Cryptosporidium was detected as the biofilm matured, with the total number of C. parvum multiplying 2–3 fold during this period. With this, various Cryptosporidium developmental stages (sporozoites, trophozoites, type I and II meronts) were identified from the biofilm. This is the first study demonstrating that biofilms not only serve as an environmental reservoir for oocysts, but are also capable of supporting the multiplication of Cryptosporidium over time in an aquatic environment.
登录
查看更多内容
影响因子：
5.7
作者：
Barraud N;Storey MV;Moore ZP;Webb JS;Rice SA;Kjelleberg S
通讯作者：
Kjelleberg S
影响因子：
2.4
作者：
Matsubayashi, M.;Ando, H.;Sasai, K.
通讯作者：
Sasai, K.
影响因子：
6.7
作者：
Declerck, Priscilla;Behets, Jonas;Ollevier, Frans
通讯作者：
Ollevier, Frans
影响因子：
2.4
作者：
Borowski, H.;Thompson, R. C. A.;Clode, P. L.
通讯作者：
Clode, P. L.
影响因子：
2.1
作者：
Boxell, Annika;Hijjawi, Nawal;Ryan, Una
通讯作者：
Ryan, Una