High Hydrostatic Pressure Inducible Trimethylamine N-Oxide Reductase Improves the Pressure Tolerance of Piezosensitive Bacteria Vibrio fluvialis.
High Hydrostatic Pressure Inducible Trimethylamine N-Oxide Reductase Improves the Pressure Tolerance of Piezosensitive Bacteria Vibrio fluvialis.
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高静水压诱导三甲胺氮氧化物还原酶提高压敏细菌河流弧菌的耐压性
DOI：
10.3389/fmicb.2017.02646
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发表时间：
2017
影响因子：
5.2
通讯作者：
Wu LF
中科院分区：
文献类型：
--
作者：
Yin QJ;Zhang WJ;Qi XQ;Zhang SD;Jiang T;Li XG;Chen Y;Santini CL;Zhou H;Chou IM;Wu LF
高静水压（HHP）对细胞过程产生严重影响，包括受损的细胞分裂，废除运动性和影响酶活性。转录组学和蛋白质组学分析表明，细菌切换参与多种能量代谢途径的基因的表达，以科普HHP。我们通过提供适当的底物来寻找改变细菌代谢的证据，这些底物可能在高压下对细菌的生活方式产生有益的影响。从中国南海海域分离到一株对压力敏感的河流弧菌QY 27。当以氧化三甲胺（TMAO）作为能量代谢的电子受体时，QY 27表现出了类压性表型，最适生长压力为30 MPa。拉曼光谱和生物化学分析表明，在高压条件下，TMAO代谢的效率和TMAO还原酶的活性均增加。在编码TMAO还原酶催化亚基的两个基因中，TorA的表达水平和酶活性随压力升高而上调。此外，使用CRISPR-dCas 9系统的遗传干扰测定表明，TorA对于支持QY 27的耐压性改善至关重要。我们将研究扩展到河流弧菌型菌株ATCC 33809，观察到TMAO代谢的相同表型提高了压力耐受性。这些结果提供了令人信服的证据代谢的决定性作用，在适应细菌的深海生态系统与HHP。
High hydrostatic pressure (HHP) exerts severe effects on cellular processes including impaired cell division, abolished motility and affected enzymatic activities. Transcriptomic and proteomic analyses showed that bacteria switch the expression of genes involved in multiple energy metabolism pathways to cope with HHP. We sought evidence of a changing bacterial metabolism by supplying appropriate substrates that might have beneficial effects on the bacterial lifestyle at elevated pressure. We isolated a piezosensitive marine bacterium Vibrio fluvialis strain QY27 from the South China Sea. When trimethylamine N-oxide (TMAO) was used as an electron acceptor for energy metabolism, QY27 exhibited a piezophilic-like phenotype with an optimal growth at 30 MPa. Raman spectrometry and biochemistry analyses revealed that both the efficiency of the TMAO metabolism and the activity of the TMAO reductase increased under high pressure conditions. Among the two genes coding for TMAO reductase catalytic subunits, the expression level and enzymatic activity of TorA was up-regulated by elevated pressure. Furthermore, a genetic interference assay with the CRISPR-dCas9 system demonstrated that TorA is essential for underpinning the improved pressure tolerance of QY27. We extended the study to Vibrio fluvialis type strain ATCC33809 and observed the same phenotype of TMAO-metabolism improved the pressure tolerance. These results provide compelling evidence for the determinant role of metabolism in the adaption of bacteria to the deep-sea ecosystems with HHP.
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影响因子：
3.2
作者：
Ansaldi, M;Jourlin-Castelli, C;Méjean, V
通讯作者：
Méjean, V
影响因子：
4.8
作者：
Baraquet, Claudine;Theraulaz, Laurence;Jourlin-Castelli, Cecile
通讯作者：
Jourlin-Castelli, Cecile
影响因子：
2.5
作者：
Al Ali, Badr;Garel, Marc;Tamburini, Christian
通讯作者：
Tamburini, Christian
DOI：
10.1111/j.1365-2672.1981.tb00873.x
发表时间：
1981-01-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
LEE, JV;SHREAD, P;BRYANT, TN
通讯作者：
BRYANT, TN
影响因子：
3.4
作者：
Frankel, RB;Bazylinski, DA;Taylor, BL
通讯作者：
Taylor, BL