qPCRtools: An R package for qPCR data processing and visualization.
qPCRtools: An R package for qPCR data processing and visualization.
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DOI：
10.3389/fgene.2022.1002704
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发表时间：
2022
影响因子：
3.7
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中科院分区：
文献类型：
--
作者：
In biological research, qPCR is a technique that is frequently used to measure gene expression levels. The calculation of gene amplification efficiency is a critical step in the processing of qPCR data since it helps to decide which method to employ to compute gene expression levels. Here, we introduce the R package qPCRtools, which enables users to analyze the efficiency of gene amplification. Additionally, this software can determine gene expression levels using one of three approaches: the conventional curve-based method, the 2−ΔΔCt method, and the SATQPCR method. The qPCRtools package produces a table with the statistical data of each method as well as a figure with a box or bar plot illustrating the results. The R package qPCRtools is freely available at CRAN (https://CRAN.R-project.org/package=qPCRtools) or GitHub (https://github.com/lixiang117423/qPCRtools/tree/main/CRAN/qPCRtools).
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影响因子：
14.9
作者：
Lievens A;Van Aelst S;Van den Bulcke M;Goetghebeur E
通讯作者：
Goetghebeur E
影响因子：
3.7
作者：
Matz MV;Wright RM;Scott JG
通讯作者：
Scott JG
影响因子：
1.7
作者：
Zhao, S;Fernald, RD
通讯作者：
Fernald, RD
影响因子：
9.3
作者：
Huggett, Jim F.;Whale, Alexandra S.;Vandesompele, Jo
通讯作者：
Vandesompele, Jo
影响因子：
3
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Mar JC;Kimura Y;Schroder K;Irvine KM;Hayashizaki Y;Suzuki H;Hume D;Quackenbush J
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Quackenbush J