Experimental autoimmune labyrinthitis: assessment of molecular size of autoantigens in fractions of inner ear proteins eluted on the Mini Whole Gel Eluter.
Experimental autoimmune labyrinthitis: assessment of molecular size of autoantigens in fractions of inner ear proteins eluted on the Mini Whole Gel Eluter.
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实验性自身免疫性迷路炎：评估在小型全凝胶洗脱仪上洗脱的内耳蛋白组分中自身抗原的分子大小。
DOI：
10.1080/003655402/000028061
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发表时间：
2002
影响因子：
1.4
通讯作者：
S. Tomiyama
中科院分区：
文献类型：
--
作者：
S. Tomiyama
为了确定实验性自身免疫性迷路炎的致病自身抗原的分子大小，将内耳抗原粗品分离成14个组分，并用迷你全凝胶洗脱剂进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法鉴定其组成分子。平均总蛋白回收率约为66%。55-65 kDa的致敏蛋白诱导的浸润性细胞数最多，38-45 kDa的致敏蛋白诱导的炎症细胞数次之。这些结果表明，38-45 kDa和55-65 kDa蛋白是实验性自身免疫性迷路炎的致病自身抗原。
In order to determine the molecular size of the causative autoantigens of experimental autoimmune labyrinthitis, crude inner ear antigen was separated into 14 fractions and the constituent molecules were identified by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Mini Whole Gel Eluter. The mean total protein recovery was approximately 66%. Sensitization with 55-65 kDa proteins induced the highest number of infiltrating cells among the fractions and sensitization with 38-45 kDa proteins induced the second highest number of inflammatory cells. These results suggest that 38-45 kDa and 55-65 kDa proteins are the causative autoantigens of experimental autoimmune labyrinthitis.
DOI：
10.1016/s0925-4439(00)00101-0
发表时间：
2001-03-26
影响因子：
6.2
作者：
Ikezono, T;Omori, A;Yagi, T
通讯作者：
Yagi, T