Increased number of Tc17 and correlation with Th17 cells in patients with immune thrombocytopenia.
Increased number of Tc17 and correlation with Th17 cells in patients with immune thrombocytopenia.
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免疫性血小板减少症患者 Tc17 数量增加及其与 Th17 细胞的相关性
DOI：
10.1371/journal.pone.0026522
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hou M
中科院分区：
文献类型：
--
作者：
Hu Y;Ma DX;Shan NN;Zhu YY;Liu XG;Zhang L;Yu S;Ji CY;Hou M
背景分泌IL-17的CD 8 + T细胞（Tc 17亚群）最近被定义为参与自身免疫性疾病发病机制的效应T细胞亚群。Tc 17在免疫性血小板减少症（ITP）病理生理学中的作用及其与Th 17细胞的相关性仍不清楚。设计与方法我们研究了47例ITP患者（20例新诊断，27例完全缓解）和34例健康对照。通过流式细胞术评价产生IL-17的CD 3 + CD 8+细胞（Tc 17）和产生IL-17的CD 3 + CD 8 −细胞（Th 17），并表示为占CD 3+细胞总数的百分比。通过改良的单克隆抗体特异性固定血小板抗原来测定特异性抗血小板糖蛋白（GP）GPIIb/IIIa和/或GPIb/IX自身抗体。分离ITP患者的外周血单个核细胞，在0、0.25、0.5或1 μmol/L地塞米松存在下孵育72 h，并收集以通过流式细胞术分析检测Tc 17和Th 17细胞。结果IL-17表达于CD 3 + CD 8 −和CD 3 + CD 8 + T细胞。初治患者Tc 17和Th 17细胞百分比较对照组明显升高，临床治疗后Tc 17细胞百分比下降。初治患者Th 17 ∶ Tc 17比值明显低于对照组，完全缓解患者Th 17 ∶ Tc 17比值明显高于对照组。对照组Tc 17与Th 17细胞之间存在显著正相关，而ITP患者Tc 17与Th 17细胞之间无相关性。ITP患者的Tc 17与CD 8 ∶ CD 4比值及CD 8+细胞呈正相关。Tc 17在自身抗体阴性患者中的频率略高于自身抗体阳性患者。此外，在ITP患者中，随着培养基中地塞米松浓度的增加，Tc 17和Th 17细胞百分比均降低。结论Tc 17和Th 17亚群参与了ITP的免疫病理过程。阻断异常增加的Tc 17可能是治疗ITP的合理策略。
Background IL-17-secreting CD8+ T cells (Tc17 subset) have recently been defined as a subpopulation of effector T cells implicated in the pathogenesis of autoimmune diseases. The role of Tc17 and correlation with Th17 cells in the pathophysiology of immune thrombocytopenia (ITP) remain unsettled. Design and Methods We studied 47 ITP patients (20 newly-diagnosed and 27 with complete response) and 34 healthy controls. IL-17-producing CD3+CD8+ cells (Tc17) and IL-17-producing CD3+CD8− cells (Th17) were evaluated by flow cytometry and expressed as a percentage of the total number of CD3+ cells. Specific anti-platelet glycoprotein (GP) GPIIb/IIIa and/or GPIb/IX autoantibodies were measured by modified monoclonal antibody specific immobilization of platelet antigens. Peripheral blood mononuclear cells of ITP patients were isolated, incubated in the presence of 0, 0.25, 0.5, or 1 µmol/L of dexamethasone for 72 h, and collected to detect Tc17 and Th17 cells by flow cytometric analysis. Results IL-17 was expressed on CD3+CD8− and CD3+CD8+ T cells. The percentages of Tc17 and Th17 cells in newly-diagnosed patients were significantly elevated compared to controls, and Tc17 was decreased after clinical treatment. The Th17∶Tc17 ratio was significantly lower in newly-diagnosed patients compared with controls, and was increased in patients who had complete response. There was a significantly positive correlation between Tc17 and Th17 cells in the control group, but not in the ITP patients. A positive correlation existed between Tc17 and the CD8∶CD4 ratio, as well as CD8+ cells in patients with ITP. The frequencies of Tc17 were marginally higher in autoantibody-negative patients than autoantibody-positive patients. Moreover, both Tc17 and Th17 cell percentages decreased as the concentration of dexamethasone in the culture media increased in ITP patients. Conclusions Tc17 and the Th17 subset are involved in the immunopathology of ITP. Blocking the abnormally increased number of Tc17 may be a reasonable therapeutic strategy for ITP.
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影响因子：
64.5
作者：
Ivanov, Ivaylo I.;McKenzie, Brent S.;Littman, Dan R.
通讯作者：
Littman, Dan R.
影响因子：
4.3
作者：
Henriques, Ana;Ines, Luis;Paiva, Artur
通讯作者：
Paiva, Artur
DOI：
10.4049/jimmunol.0801814
发表时间：
2009-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hamada H;Garcia-Hernandez Mde L;Reome JB;Misra SK;Strutt TM;McKinstry KK;Cooper AM;Swain SL;Dutton RW
通讯作者：
Dutton RW
影响因子：
5.4
作者：
Huber, Magdalena;Heink, Sylvia;Lohoff, Michael
通讯作者：
Lohoff, Michael
影响因子：
32.4
作者：
Yang, Xuexian O.;Pappu, Bhanu P.;Dong, Chen
通讯作者：
Dong, Chen