Changes to the sebum lipidome upon COVID-19 infection observed via rapid sampling from the skin.
Changes to the sebum lipidome upon COVID-19 infection observed via rapid sampling from the skin.
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通过皮肤快速取样观察 COVID-19 感染后皮脂脂质体的变化。
DOI：
10.1016/j.eclinm.2021.100786
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发表时间：
2021-03
影响因子：
15.1
通讯作者：
Bailey M
中科院分区：
文献类型：
--
作者：
Spick M;Longman K;Frampas C;Lewis H;Costa C;Walters DD;Stewart A;Wilde M;Greener D;Evetts G;Trivedi D;Barran P;Pitt A;Bailey M
2019冠状病毒病大流行导致了对检测的前所未有的需求-用于诊断和预后-以及调查疾病对宿主代谢的影响。皮脂采样有可能通过观察病毒对我们的影响来支持这两种需求，而不是寻找病毒本身。在这项初步研究中，通过纱布拭子从67名住院患者（30名COVID-19阳性患者和37名COVID-19阴性患者）中采集皮脂样本。使用液相色谱质谱法进行脂质组学分析，鉴定了998个可重复的特征。将单变量和多变量统计分析应用于所得特征集。COVID-19阳性参与者的血脂水平降低，表明血脂异常;甘油三酯和神经酰胺的p值分别为0.022和0.015，效应量分别为0.44和0.57。偏最小二乘判别分析显示，COVID-19阳性和阴性参与者的分离敏感性为57%，特异性为68%，在控制混杂合并症时分别提高到79%和83%。COVID-19使代谢的许多领域失调;在这项工作中，我们表明皮肤脂质组可以被添加到列表中。鉴于样本可以快速无痛地提供，我们得出结论，皮脂值得将来考虑用于临床采样。作者感谢EPSRC Impact Acceleration Account为样本收集和处理提供的资金，以及EPSRC Fellowship Funding EP/R 031118/1，University of Surrey和BBSRC BB/T002212/1。质谱法由EP/P001440/1资助。
The COVID-19 pandemic has led to an unprecedented demand for testing - for diagnosis and prognosis - as well as for investigation into the impact of the disease on the host metabolism. Sebum sampling has the potential to support both needs by looking at what the virus does to us, rather than looking for the virus itself. In this pilot study, sebum samples were collected from 67 hospitalised patients (30 COVID-19 positive and 37 COVID-19 negative) by gauze swab. Lipidomics analysis was carried out using liquid chromatography mass spectrometry, identifying 998 reproducible features. Univariate and multivariate statistical analyses were applied to the resulting feature set. Lipid levels were depressed in COVID-19 positive participants, indicative of dyslipidemia; p-values of 0·022 and 0·015 were obtained for triglycerides and ceramides respectively, with effect sizes of 0·44 and 0·57. Partial Least Squares-Discriminant Analysis showed separation of COVID-19 positive and negative participants with sensitivity of 57% and specificity of 68%, improving to 79% and 83% respectively when controlled for confounding comorbidities. COVID-19 dysregulates many areas of metabolism; in this work we show that the skin lipidome can be added to the list. Given that samples can be provided quickly and painlessly, we conclude that sebum is worthy of future consideration for clinical sampling. The authors acknowledge funding from the EPSRC Impact Acceleration Account for sample collection and processing, as well as EPSRC Fellowship Funding EP/R031118/1, the University of Surrey and BBSRC BB/T002212/1. Mass Spectrometry was funded under EP/P001440/1.
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影响因子：
15.1
作者：
Ruszkiewicz DM;Sanders D;O'Brien R;Hempel F;Reed MJ;Riepe AC;Bailie K;Brodrick E;Darnley K;Ellerkmann R;Mueller O;Skarysz A;Truss M;Wortelmann T;Yordanov S;Thomas CLP;Schaaf B;Eddleston M
通讯作者：
Eddleston M
影响因子：
6.2
作者：
Ranninger, Christina;Schmidt, Lukas E.;Huber, Christian G.
通讯作者：
Huber, Christian G.
DOI：
10.14132/j.cnki.1673-5439.2020.06.001
发表时间：
2020-03-11
影响因子：
1.7
作者：
Chen, Fang;Huang, Shi-Yun;Wu, Peng
通讯作者：
Wu, Peng
影响因子：
3.4
作者：
Brown KJ;Formolo CA;Seol H;Marathi RL;Duguez S;An E;Pillai D;Nazarian J;Rood BR;Hathout Y
通讯作者：
Hathout Y
影响因子：
4.1
作者：
Shui, Guanghou;Cheong, Wei Fun;Wenk, Markus R.
通讯作者：
Wenk, Markus R.