Huatanjiangqi Capsule Upregulating NRF2 and MRP1 Expression in Chronic Obstructive Pulmonary Disease in Rats
Huatanjiangqi Capsule Upregulating NRF2 and MRP1 Expression in Chronic Obstructive Pulmonary Disease in Rats
复制标题
化痰降气胶囊上调慢性阻塞性肺疾病大鼠NRF2和MRP1表达
DOI：
10.1007/s11094-018-1771-z
复制
发表时间：
2018-05
影响因子：
0.9
通讯作者：
Fang Wei
中科院分区：
文献类型：
--
作者：
Xu Xiaoya;Chen Yajun;Zhang Min;Wang Dianlei;Wu Jie;Yao Zhaomin;Wu Qingqing;Fang Wei
化痰降气胶囊治疗慢性阻塞性肺疾病（COPD）临床疗效显著。MRP 1的功能活性降低与COPD的发展有关。然而，HTJQ导致MRP 1上调的机制尚不清楚。本研究旨在探讨HTJQ对慢性阻塞性肺疾病（COPD）大鼠肺组织Nrf 2和MRP 1表达的影响。采用香烟烟雾加脂多糖气管滴注诱导大鼠COPD模型。然后检测肺组织中Nrf 2、HO-1和MRP 1 mRNA表达及其蛋白质产生。模型组上述指标与对照组比较有显著性差异（p< 0.05）。模型组高、低剂量治疗组肺功能明显增强（均P < 0.05），肺组织炎性细胞和杯状细胞减少。HTJQ低、高剂量组Nrf 2、HO-1、MRP 1 mRNA和蛋白表达水平均明显升高（p< 0.05）。HTJQ可上调COPD模型大鼠肺组织Nrf 2和MRP 1的表达。HTJQ上调MRP 1表达的机制可能与Nrf 2有关。
The therapy of Huatanjiangqi capsule (HTJQ) has been used in the treatment of chronic obstructive pulmonary disease (COPD) clinically, with remarkable benefits. A lower functional activity of MRP1 is related to COPD development. However, the mechanism that contributes to MRP1 up-regulation by HTJQ was unclear. This study aimed to investigate the effects of HTJQ on the expression of Nrf2 and MRP1 in COPD rats. Rats were exposed to cigarette smoke plus lipopolysaccharide tracheal instillation to induce a COPD model. Then, Nrf2, HO-1, and MRP1 mRNA expression and their protein production in lung tissues were examined. The model group showed significant changes in the above assessments as compared to those of the control group (p< 0.05). The lung function of model group treated with low/high dose of HTJQ was significantly strengthened (bothp< 0.05), and the inflammatory cells and goblet cells were decreased in lung tissues. Treatment with low/high dose of HTJQ resulted in obvious increase in Nrf2, HO-1, and MRP1 mRNA and protein expression levels (p< 0.05). HTJQ could up-regulate Nrf2 and MRP1 expression in lung tissues of COPD model rats. The mechanism by which HTJQ up-regulates MRP1 expression may be associated with Nrf2.
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DOI：
10.1006/bbrc.1997.7017
发表时间：
1997-07-30
影响因子：
3.1
作者：
Paslaru, L;Trigon, S;Morange, M
通讯作者：
Morange, M
影响因子：
2.5
作者：
R. Pauwels;A. Buist;P. Ma;C. Jenkins;S. Hurd
通讯作者：
R. Pauwels;A. Buist;P. Ma;C. Jenkins;S. Hurd
影响因子：
3.9
作者：
Maher, JM;Cheng, XG;Klaassen, CD
通讯作者：
Klaassen, CD
影响因子：
6.7
作者：
Xi Chen;X. Guan;Xiaohong Peng;Zhi-lei Cui;C-Y. Luan;Xue-Jun Guo
通讯作者：
Xi Chen;X. Guan;Xiaohong Peng;Zhi-lei Cui;C-Y. Luan;Xue-Jun Guo
影响因子：
9.6
作者：
Hoi Nam Tse;Raiteri, Luca;Tseng, Cee Zhung Steven
通讯作者：
Tseng, Cee Zhung Steven