Extension of long cellular processes of hepatic stellate cells cultured on extracellular type I collagen gel by microtubule assembly: observation utilizing time-lapse video-microscopy.
Extension of long cellular processes of hepatic stellate cells cultured on extracellular type I collagen gel by microtubule assembly: observation utilizing time-lapse video-microscopy.
复制标题
通过微管组装在细胞外 I 型胶原凝胶上培养的肝星状细胞的长细胞过程的延伸：利用延时视频显微镜观察。
DOI：
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发表时间：
1997
影响因子：
1.5
通讯作者：
H. Senoo
中科院分区：
文献类型：
--
作者：
M. Miura;M. Sato;I. Toyoshima;H. Senoo
在新鲜制备的 I 型胶原凝胶上或其中作为培养基培养的肝星状细胞被诱导延长长的细胞过程。使用视频增强光学显微镜监测细胞过程的延伸。细胞过程似乎沿着细胞外 I 型胶原纤维延伸。在 I 型胶原凝胶上培养过夜后，一旦延长的细胞突起会因秋水仙碱或细胞松弛素 B 的细胞骨架降解而收缩。使用蛋白激酶抑制剂、除草霉素 A 或十字孢菌素或磷脂酰肌醇 3-激酶抑制剂、渥曼青霉素处理也会使细胞突起收缩。秋水仙碱、除草霉素 A、十字孢菌素或渥曼青霉素的作用非常显着，细胞最终在几小时内变成圆形，这在 4 摄氏度冷处理后也能看到。细胞松弛素 B 也随时间依赖性地收缩延长的细胞过程。这些结果表明，培养的星状细胞通过细胞表面与I型胶原原纤维结合、随后蛋白质或磷脂酰肌醇磷酸化、最后F-肌动蛋白和微管组装而被诱导延长细胞过程。延长的长细胞过程似乎反映了肝星状细胞的体内结构，并提出了细胞过程延长和维持的分子机制。
Hepatic stellate cells cultured on or in freshly prepared type I collagen gel as a substratum were induced to elongate long cellular processes. The extension of the cellular processes was monitored by using video-enhanced optical microscopy. The cellular processes seemed to extend along the extracellular type I collagen fibers. Once extended cellular processes after overnight culture on type I collagen gel were retracted by cytoskeleton degradation with colchicine or cytochalasin B. The cellular processes were also retracted by treatment with protein kinase inhibitor, herbimycin A or staurosporin, or with phosphatidylinositol 3-kinase inhibitor, wortmannin. The effects of colchicine, herbimycin A, staurosporin, or wortmannin were drastic, and the cells were finally changed to a round shape within a few hours, as seen also after cold-treatment at 4 degrees C. Cytochalasin B also time-dependently retracted the extended cellular processes. These results indicated that the cultured stellate cells were induced to elongate cellular processes by cell surface binding to type I collagen fibrils, followed by protein or phosphatidylinositol phosphorylation and finally F-actin and microtubule assembly. Extended long cellular processes seem to reflect the in vivo structure of hepatic stellate cells, and molecular mechanism for the extension and maintenance of cellular processes was proposed.
DOI：
10.1073/pnas.88.19.8392
发表时间：
1991-10-01
影响因子：
11.1
作者：
KORNBERG, LJ;EARP, HS;JULIANO, RL
通讯作者：
JULIANO, RL
胶原蛋白中α2β1整合素四肽识别序列的鉴定。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Staatz,WD;Fok,KF;Zutter,MM;Adams,SP;Rodriguez,BA;Santoro,SA
通讯作者：
Santoro,SA
细胞外基质调节细胞功能。
DOI：
10.1007/bf00861587
发表时间：
1993
期刊：
Pediatric nephrology (Berlin, Germany)
影响因子：
--
作者：
Schnaper,HW;Kleinman,HK
通讯作者：
Kleinman,HK