Nogo-66 Inhibits the Dye-coupling of Astrocytic Gap Junctions In Vitro
Nogo-66 Inhibits the Dye-coupling of Astrocytic Gap Junctions In Vitro
复制标题
Nogo-66 在体外抑制星形胶质细胞间隙连接的染料偶联
DOI：
10.1007/s11064-011-0460-z
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发表时间：
2011-04
影响因子：
4.4
通讯作者：
Ju, Gong
中科院分区：
文献类型：
--
作者：
Wang, Yazhou;Wu, Yin;Liu, Mengdong;Wang, Jian;Ju, Gong
星形胶质细胞之间通过缝隙连接的通讯对于维持中枢神经系统神经元外微环境的动态平衡至关重要。星形胶质细胞缝隙连接功能障碍与许多脑部疾病有关。我们以前的研究表明，Nogo-66受体在大鼠小脑和垂体后叶的神经胶质细胞缝隙连接处有一个新的共定位。本研究旨在探讨Nogo-66对体外培养的神经胶质细胞缝隙连接是否具有调节作用。我们证实了Nogo-66受体与Cx43在培养的星形胶质细胞中共定位，并用髓鞘提取物或Nogo-66-FC条件培养液刺激星形胶质细胞。最后，我们表达并纯化了一种功能有效的GST-Nogo-66多肽。采用荧光黄转移实验测定缝隙结通透性。结果表明，与相应的对照相比，三种处理均能显著抑制荧光黄的传播。因此，本研究显示了Nogo-66对星形胶质细胞缝隙连接通透性的一种新的抑制作用，提示Nogo-66受体可能在神经胶质细胞缝隙连接的调节中发挥作用。
Communication between astrocytes via the gap junction is crucial for maintaining homeostasis of the extra-neuronal microenvironment of the central nervous system. Dysfunction of astrocytic gap junctions is involved in many brain disorders. Our previous studies demonstrated a novel co-localization of Nogo-66 receptor at glial gap junctions in rat cerebellum and posterior pituitary. The present study was aimed at exploring whether Nogo-66 can modulate glial gap junctions in vitro. We confirmed the co-localization of Nogo-66 receptor with Cx43 in cultured astrocytes, and stimulated astrocytes with myelin extracts, or Nogo-66-Fc conditioned medium. Finally, we expressed and purified a functionally effective GST-Nogo-66 peptide. Lucifer yellow transfer assay was adopted to measure the gap junction permeability. The results showed that the spreading of Lucifer yellow was inhibited significantly by all three treatments as compared with their corresponding controls. Therefore, this study shows a novel inhibitory effect of Nogo-66 on the permeability of astrocytic gap junctions, suggesting a presumable role of Nogo-66 receptor in modulating the glial gap junction.
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影响因子：
3.7
作者：
Wang B;Xiao Z;Chen B;Han J;Gao Y;Zhang J;Zhao W;Wang X;Dai J
通讯作者：
Dai J
大鼠垂体细胞间隙连接处的 Nogo-66 受体
影响因子：
1.7
作者：
通讯作者：
--
影响因子：
5.2
作者：
B. Zörner;M. Schwab
通讯作者：
B. Zörner;M. Schwab
影响因子：
25
作者：
Yamashita, T;Tohyama, M
通讯作者：
Tohyama, M
影响因子：
6.2
作者：
Yongmei Zhao;Mark A. Rivieccio;S. Lutz;E. Scemes;C. Brosnan
通讯作者：
Yongmei Zhao;Mark A. Rivieccio;S. Lutz;E. Scemes;C. Brosnan