The influence of substrate elastic modulus on retinal pigment epithelial cell phagocytosis.
The influence of substrate elastic modulus on retinal pigment epithelial cell phagocytosis.
复制标题
DOI：
10.1016/j.jbiomech.2014.06.021
复制
发表时间：
2014-09-22
影响因子：
2.4
通讯作者：
Foster, William J.
中科院分区：
文献类型：
--
作者：
Boochoon, Kieran S.;Manarang, Joseph C.;Davis, Joshua T.;McDermott, Alison M.;Foster, William J.
为了更好地理解，如果一个复杂的过程，如吞噬作用是由基板刚度的影响，我们研究了基板的弹性模量对视网膜色素上皮（RPE）细胞系ARPE-19中的吞噬作用。RPE细胞位于视网膜正下方的布鲁赫膜上，并吞噬脱落的感光细胞外节。已知Bruch膜硬度随年龄增加一个数量级，因此，本研究在解释年龄相关性黄斑变性的视网膜变化方面具有潜在相关性。将ARPE-19细胞铺在不同弹性模量的层粘连蛋白包被的聚丙烯酰胺基质上。培养14天后，将悬浮在PBS中的乳胶荧光珠溶液置于每个孔中。孵育4小时后，进行流式细胞术以确定吞噬珠粒的细胞数。吞噬珠粒的ARPE-19细胞的数量随着基质弹性模量的增加而连续减少（p=0.0135），并且发现这是线性关系（斜率=-0.03305 ± 0.01104，R2 =0.4726/10，000个细胞）。我们的研究结果表明，RPE细胞显示减少吞噬作用时，更坚定的基板上生长，因此，在老年人的眼睛，其中布鲁赫膜是僵硬的，RPE细胞可能会表现出减少吞噬作用。RPE细胞的吞噬功能受损可能导致感光细胞外节代谢受损和黄斑变性的发展。材料硬度可能是神经疗法（包括视网膜修复术和干细胞疗法）开发中的关键参数。
To better understand if a complex process such as phagocytosis is influenced by substrate stiffness, we investigated the influence of substrate elastic modulus on phagocytosis in the retinal pigment epithelial (RPE) cell line ARPE-19. RPE cells lie on Bruch’s membrane, directly under the retina, and phagocytose the shed photoreceptor outer segments. Bruch’s membrane is known to increase in stiffness by an order of magnitude with age and thus, this study has potential relevance in explaining retinal changes in age-related macular degeneration. ARPE-19 cells were plated on laminin-coated polyacrylamide substrates of varying elastic modulus. After 14 days in culture, a solution of latex fluorescent beads suspended in PBS was placed in each well. After an incubation time of 4 hours, flow cytometry was performed to determine the number of cells that phagocytosed a bead. The number of ARPE-19 cells that phagocytosed a bead decreased continuously as a function of increasing substrate elastic modulus (p=0.0135), and this was found to be a linear relationship (slope=−0.03305 ± 0.01104, R2 =0.4726 per 10,000 cells). Our results suggest that RPE cells display decreased phagocytosis when grown on firmer substrates, and thus, RPE cells in older eyes, in which Bruch’s membrane is stiffer, may demonstrate decreased phagocytosis. Impaired phagocytosis by RPE cells may contribute to impaired metabolism of photoreceptor outer segments and to development of macular degeneration. Material stiffness may be a critical parameter in the development of neural therapies, including retinal prosthetics and stem cell therapies.
登录
查看更多内容
DOI：
10.1038/eye.1987.35
发表时间：
1987-01-01
期刊：
EYE-TRANSACTIONS OF THE OPHTHALMOLOGICAL SOCIETIES OF THE UNITED KINGDOM
影响因子：
--
作者：
FISHER, RF
通讯作者：
FISHER, RF
影响因子：
4.4
作者：
Raghunathan, Vijay Krishna;Morgan, Joshua T.;Russell, Paul
通讯作者：
Russell, Paul
影响因子：
4.4
作者：
Davis, Joshua T.;Wen, Qi;Foster, William J.
通讯作者：
Foster, William J.
影响因子：
17.8
作者：
Guidry, C
通讯作者：
Guidry, C
影响因子：
8
作者：
Provenzano, P. P.;Inman, D. R.;Eliceiri, K. W.;Keely, P. J.
通讯作者：
Keely, P. J.