Homing of endogenous bone marrow mesenchymal stem cells to rat infarcted myocardium via ultrasound-mediated recombinant SDF-1α adenovirus in microbubbles.
Homing of endogenous bone marrow mesenchymal stem cells to rat infarcted myocardium via ultrasound-mediated recombinant SDF-1α adenovirus in microbubbles.
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通过超声介导的微泡中的重组 SDF-1α 腺病毒将内源性骨髓间充质干细胞归巢到大鼠梗塞心肌
DOI：
10.18632/oncotarget.23068
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发表时间：
2018-01-02
期刊：
影响因子：
--
通讯作者：
Guan L
中科院分区：
文献类型：
--
作者：
Su G;Liu L;Yang L;Mu Y;Guan L
干细胞可以促进心肌再生，加速新血管的形成。因此，干细胞移植是治疗急性心肌梗死(AMI)的一种很有前途的方法。干细胞通过趋化作用自发地定位于梗死心肌，其中基质细胞衍生因子(SDF-1α)被证明是最重要的趋化因子之一。然而，自发分泌的SDF-1α寿命较短，不能满足组织修复的需要。本研究将携带sdf-1α基因的腺病毒负载到微泡载体上，并通过超声靶向破坏微泡将其释放到急性心肌梗死大鼠体内。通过检测骨髓间充质干细胞从外周血向心肌梗死区归巢的数量，探讨SDF-1α过表达诱导的骨髓间充质干细胞在梗死区心肌内自体移植的可能性。转染后外周血中sdf-1α浓度显著升高，外周血和梗死区骨髓间充质干细胞数量显著增加。进一步分析表明，归巢骨髓间充质干细胞的数量随着SDF-1α表达的增加而增加。结论：超声介导外源性α基因导入心肌梗死大鼠心肌梗死后可有效促进内源性骨髓间充质干细胞归巢入心。此外，归巢干细胞的数量受sdf-1α表达水平的控制。
Stem cells can promote myocardial regeneration and accelerate the formation of new blood vessels. As such, transplanted stem cells represent a promising treatment modality for acute myocardial infarction (AMI). Stem cells spontaneously home to the infarcted myocardium using chemotaxis, in which the stromal cell-derived factor (SDF-1α) has been shown to be one of the most important chemokines. However, spontaneously secreted SDF-1α is short-lived, and therefore does not meet the needs of tissue repair. In this study, adenoviruses carrying SDF-1α genes were loaded on microbubble carriers and the adenoviruses were released into AMI rats by ultrasound targeted microbubble destruction. The possibility of in vivo self-transplantation of bone marrow mesenchymal stem cells (BMSCs) induced by overexpression of SDF-1α in the infarcted myocardium was explored by detecting the number of BMSCs homing from the peripheral blood to the myocardial infarcts. The concentration of SDF-1α in peripheral blood was significantly higher after transfection, and the number of BMSCs was significantly higher in the peripheral blood and infarcted area. Further analyses indicated that the number of homing BMSCs increased with increased SDF-1α expression. In conclusion, our results suggest that ultrasound mediated transduction of exogenous SDF-1α genes into myocardial infarcted AMI rats can effectively promote the homing of endogenous BMSCs into the heart. Moreover, the number of homing stem cells was controlled by the level of SDF-1α expression.
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超声靶向微泡破坏通过上调 SDF-1/CXCR4 改善心肌梗塞后间充质干细胞的迁移和归巢：一项初步研究
影响因子：
4.3
作者：
Li L;Wu S;Liu Z;Zhuo Z;Tan K;Xia H;Zhuo L;Deng X;Gao Y;Xu Y
通讯作者：
Xu Y
影响因子：
5.2
作者：
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Janowska-Wieczorek, Anna
影响因子：
9.7
作者：
Nomikou, Nikolitsa;Tiwari, Poonam;McHale, Anthony P.
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McHale, Anthony P.
影响因子：
8.4
作者：
Avivi, S;Gedanken, A
通讯作者：
Gedanken, A
影响因子：
37.8
作者：
Kraitchman, DL;Tatsumi, M;Bulte, JWM
通讯作者：
Bulte, JWM