The Correlation Study between Plasma Aβ Proteins and Cerebrospinal Fluid Alzheimer's Disease Biomarkers
The Correlation Study between Plasma Aβ Proteins and Cerebrospinal Fluid Alzheimer's Disease Biomarkers
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血浆Aβ蛋白与脑脊液阿尔茨海默病生物标志物的相关性研究
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
S. A. Park
中科院分区：
文献类型：
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作者：
H. Shin;Seung;Saeromi Kim;Hyung;W. Chae;S. A. Park
背景与目的阿尔茨海默病（Alzheimer's disease，AD）是以β淀粉样蛋白（amyloid-β proteins，Aβ）积累为特征的疾病。本研究旨在探讨AD患者和对照组血浆Aβ40和Aβ42水平与脑脊液（CSF）中Aβ42、总tau蛋白（tTau）和磷酸化tau 181（pTau 181）水平的相关性，以进一步了解血浆Aβ蛋白水平的特点。方法连续入选44例AD患者和47例对照者。使用市售夹心酶联免疫吸附试验（ELISA）试剂盒测量Aβ40和Aβ42的血浆水平。采用INNOTEST ELISA试剂盒检测相应CSF中Aβ42、tTau和pTau 181的浓度。此外，测量血清和CSF中的白蛋白水平，并获得白蛋白比率以检查血脑屏障的完整性。结果AD患者CSF中Aβ42水平显著降低，tTau和pTau 181水平显著升高。AD患者血浆Aβ42水平显著低于对照组（p=0.007），而Aβ40/Aβ42比值显著高于对照组（p<0.001）。总体血浆Aβ42水平与CSF Aβ42水平呈正相关（p=0.001），但与CSF中的其他水平无关。在亚组分析中，对照组CSF Aβ 42与血浆Aβ42和Aβ40均呈正相关。而AD组血浆和CSF中Aβ蛋白含量无明显相关性。结论正常人血浆Aβ42和Aβ40水平与脑脊液Aβ42水平密切相关，而AD患者血浆Aβ42和Aβ40水平与脑脊液Aβ42水平无关。我们的结果表明，血浆Aβ40和CSF Aβ42水平之间的相关性在AD中受到干扰。
Background and Purpose Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β proteins (Aβ). In this study we explored the correlation of plasma Aβ40 and Aβ42 concentrations with Aβ42, total tau (tTau) and phosphorylated tau 181 (pTau181) levels in cerebrospinal fluid (CSF) in AD and control subjects to further understand the characteristics of plasma Aβ proteins levels. Methods The consecutive subjects (44 AD and 47 controls) in this study were recruited. The plasma levels of Aβ40 and Aβ42 were measured using a commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kits. And the corresponding CSFs were analyzed in terms of Aβ42, tTau and pTau181 concentrations using INNOTEST ELISA kits. Further, the albumin levels were measured both in serum and CSF and albumin ratio was obtained to check the integrity of blood-brain barrier. Results CSF Aβ42 concentrations were significantly decreased while tTau and pTau181 levels were significantly increased in AD subjects. The plasma levels of Aβ42 were significantly lower (p=0.007), while the Aβ40/Aβ42 ratio was significantly higher (p<0.001) in AD patients than in controls. The overall plasma Aβ42 levels showed a positive correlation with those of CSF Aβ42 (p=0.001), but not with the others in CSF. In subgroup analysis, the CSF Aβ42 demonstrated positive correlation with not only plasma Aβ42 but also Aβ40 levels in controls. However, no significant relationship was noted between plasma and CSF Aβ proteins in AD group. Conclusions The plasma Aβ42 and Aβ40 concentrations were shown to have a close relationship with CSF Aβ42 levels in controls, but not in AD subjects. Our results suggest that the correlation between plasma Aβ40 and CSF Aβ42 levels is perturbed in AD.
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人血浆中的β-淀粉样动力学。
影响因子：
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作者：
Huang Y;Potter R;Sigurdson W;Kasten T;Connors R;Morris JC;Benzinger T;Mintun M;Ashwood T;Ferm M;Budd SL;Bateman RJ
通讯作者：
Bateman RJ
影响因子：
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作者：
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Grodstein, Francine
DOI：
10.3233/jad-2011-101977
发表时间：
2011
期刊：
Journal of Alzheimer's disease : JAD
影响因子：
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作者：
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Sachdev PS
影响因子：
29
作者：
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3
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