Arabidopsis PCaP2 Functions as a Linker Between ABA and SA Signals in Plant Water Deficit Tolerance.
Arabidopsis PCaP2 Functions as a Linker Between ABA and SA Signals in Plant Water Deficit Tolerance.
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拟南芥 PCaP2 在植物耐缺水性中充当 ABA 和 SA 信号之间的链接器
DOI：
10.3389/fpls.2018.00578
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发表时间：
2018
影响因子：
5.6
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Wang X;Wang Y;Wang L;Liu H;Zhang B;Cao Q;Liu X;Lv Y;Bi S;Zhang S;He M;Tang S;Yao S;Wang C
水分胁迫对植物的生长、发育和生产力有重要影响。然而，参与耐旱性的串扰网络还没有得到很好的理解。拟南芥PCaP 2是一种质膜结合蛋白。在本研究中，我们采用qRT-PCR和β-葡萄糖醛酸酶（GUS）组织化学染色证明PCaP 2表达强烈诱导根，子叶，真叶，侧根，和整个植物在水分亏缺条件下。与野生型（WT）植物相比，PCaP 2过表达（PCaP 2-OE）植物在种子萌发、幼苗生长和植物存活状态方面表现出增强的水分亏缺耐受性。相反，PCaP 2的突变和PCaP 2-RNAi的降低使植物对水分亏缺更敏感。此外，PCaP 2-RNAi和pcap 2幼苗在正常条件下表现出较短的根毛和较低的相对含水量，这些表型在水分亏缺下加剧。此外，外源脱落酸（阿坝）和水杨酸（SA）处理强烈诱导PCaP 2的表达，与pcap 2和PCaP 2-RNAi的敏感表型相反，PCaP 2-OE植株表现出对外源阿坝和SA处理的不敏感。众所周知，SNF 1相关激酶2s（SnRK 2s）和病程相关激酶（PRs）分别通过阿坝和SA介导的途径影响植物耐旱性。在水分亏缺、阿坝和SA处理下，PCaP 2对干旱诱导基因（RD 29 A、KIN 1和KIN 2）、ABA介导的干旱响应基因（SnRK2.2、-2.3、-2.6、ABF 1、-2、-3、-4）和SA介导的干旱响应基因（PR 1、-2、-5）的表达均有正调控作用。综上所述，我们的研究结果表明PCaP 2通过参与响应阿坝和SA信号以及调节根毛的生长，在拟南芥的水分亏缺耐性中起着重要的积极作用。这项研究为植物响应水分亏缺胁迫的潜在串扰机制提供了新的见解。
Water stress has a major influence on plant growth, development, and productivity. However, the cross-talk networks involved in drought tolerance are not well understood. Arabidopsis PCaP2 is a plasma membrane-associated Ca2+-binding protein. In this study, we employ qRT-PCR and β-glucuronidase (GUS) histochemical staining to demonstrate thatPCaP2expression was strongly induced in roots, cotyledons, true leaves, lateral roots, and whole plants under water deficit conditions. Compared with the wild type (WT) plants,PCaP2-overexpressing (PCaP2-OE) plants displayed enhanced water deficit tolerance in terms of seed germination, seedling growth, and plant survival status. On the contrary,PCaP2mutation and reduction viaPCaP2-RNAi rendered plants more sensitive to water deficit. Furthermore,PCaP2-RNAi andpcap2seedlings showed shorter root hairs and lower relative water content compared to WT under normal conditions and these phenotypes were exacerbated under water deficit. Additionally, the expression ofPCaP2was strongly induced by exogenous abscisic acid (ABA) and salicylic acid (SA) treatments.PCaP2-OE plants showed insensitive to exogenous ABA and SA treatments, in contrast to the susceptible phenotypes ofpcap2andPCaP2-RNAi. It is well-known that SNF1-related kinase 2s (SnRK2s) and pathogenesis-related (PRs) are major factors that influence plant drought tolerance by ABA- and SA-mediated pathways, respectively. Interestingly, PCaP2 positively regulated the expression of drought-inducible genes (RD29A,KIN1, andKIN2), ABA-mediated drought responsive genes (SnRK2.2,-2.3,-2.6,ABF1,-2,-3,-4), and SA-mediated drought responsive genes (PR1,-2,-5) under water deficit, ABA, or SA treatments. Taken together, our results showed that PCaP2 plays an important and positive role in Arabidopsis water deficit tolerance by involving in response to both ABA and SA signals and regulating root hair growth. This study provides novel insights into the underlying cross-talk mechanisms of plants in response to water deficit stress.
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DOI：
10.1073/pnas.0912021106
发表时间：
2009-12-15
影响因子：
11.1
作者：
Geiger, Dietmar;Scherzer, Soenke;Hedrich, Rainer
通讯作者：
Hedrich, Rainer
影响因子：
7.4
作者：
Ambrosone, Alfredo;Batelli, Giorgia;Grillo, Stefania
通讯作者：
Grillo, Stefania
影响因子：
9.4
作者：
Acharya, Biswa R.;Jeon, Byeong Wook;Assmann, Sarah M.
通讯作者：
Assmann, Sarah M.
压力记忆和干旱的不可避免的影响：一种生理观点。
影响因子：
5.6
作者：
Fleta-Soriano E;Munné-Bosch S
通讯作者：
Munné-Bosch S
影响因子：
19
作者：
Carlton, JG;Cullen, PJ
通讯作者：
Cullen, PJ