Multi-omics Comparative Analysis of Streptomyces Mutants Obtained by Iterative Atmosphere and Room-Temperature Plasma Mutagenesis.
Multi-omics Comparative Analysis of Streptomyces Mutants Obtained by Iterative Atmosphere and Room-Temperature Plasma Mutagenesis.
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通过迭代大气和室温等离子体诱变获得的链霉菌突变体的多组学比较分析
DOI：
10.3389/fmicb.2020.630309
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发表时间：
2020
影响因子：
5.2
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Liu T;Huang Z;Gui X;Xiang W;Jin Y;Chen J;Zhao J
海绵是最原始的多细胞动物，含有大量独特的微生物群落。与海绵相关的微生物，特别是放线菌，具有产生多种活性天然产物的潜力。然而，大量沉默的次级代谢基因簇在实验室培养条件下未能复活。在本研究中，迭代大气室温等离子体。采用ARTP诱变与多组学联合分析的方法对失活野生链霉菌进行了激活。本研究的理想暴露时间为75 s，以获得适当的致死率（94%）和突变阳性率（40.94%）。经过三次ARTP诱变，具有抗菌活性的突变体比例显著提高了75%。转录组分析进一步表明，与野生型菌株相比，活性突变体编码I型套索肽aborycin的差异基因表达水平有显著上升趋势，LC-MS结果证实了这一点，相对分子质量为1082.43 （[M + 2H]2+, M /z = 2164.86）。此外，对突变菌株和野生型菌株进行代谢组学比较分析发现，4个光谱或质量峰在总离子计数或提取离子电流谱上存在明显差异，每个峰对应的特定化合物对革兰氏阳性指标具有中等的抗菌活性。综上所述，我们的数据表明，结合多组学分析的ARTP治疗方法可以用于估计微生物原油代谢物的有效活性分子，而无需耗时的分离过程。
Sponges, the most primitive multicellular animals, contain a large number of unique microbial communities. Sponge-associated microorganisms, particularly actinomyces, have the potential to produce diverse active natural products. However, a large number of silent secondary metabolic gene clusters have failed to be revived under laboratory culture conditions. In this study, iterative atmospheric room-temperature plasma. (ARTP) mutagenesis coupled with multi-omics conjoint analysis was adopted to activate the inactive wild Streptomyces strain. The desirable exposure time employed in this study was 75 s to obtain the appropriate lethality rate (94%) and mutation positive rate (40.94%). After three iterations of ARTP mutagenesis, the proportion of mutants exhibiting antibacterial activities significantly increased by 75%. Transcriptome analysis further demonstrated that the differential gene expression levels of encoding type I lasso peptide aborycin had a significant upward trend in active mutants compared with wild-type strains, which was confirmed by LC-MS results with a relative molecular mass of 1082.43 ([M + 2H]2+ at m/z = 2164.86). Moreover, metabolome comparative analysis of the mutant and wild-type strains showed that four spectra or mass peaks presented obvious differences in terms of the total ion count or extracting ion current profiles with each peak corresponding to a specific compound exhibiting moderate antibacterial activity against Gram-positive indicators. Taken together, our data suggest that the ARTP treatment method coupled with multi-omics profiling analysis could be used to estimate the valid active molecules of metabolites from microbial crudes without requiring a time-consuming isolation process.
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影响因子：
9.8
作者：
Lee, Namil;Kim, Woori;Cho, Byung-Kwan
通讯作者：
Cho, Byung-Kwan
南海北部湾不同海绵中分离的放线菌多样性及其抗菌潜力
影响因子：
4.2
作者：
Liu, Tan;Wu, Shufei;Zhao, Jing
通讯作者：
Zhao, Jing
DOI：
10.1039/c29700000826
发表时间：
1970-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY D-CHEMICAL COMMUNICATIONS
影响因子：
--
作者：
BATTERSB.AR;BURNETT, AR;PARSONS, PG
通讯作者：
PARSONS, PG
影响因子：
5.7
作者：
Mullane, Kathleen M.;Gorbach, Sherwood
通讯作者：
Gorbach, Sherwood
影响因子：
4.4
作者：
Nieselt K;Battke F;Herbig A;Bruheim P;Wentzel A;Jakobsen ØM;Sletta H;Alam MT;Merlo ME;Moore J;Omara WA;Morrissey ER;Juarez-Hermosillo MA;Rodríguez-García A;Nentwich M;Thomas L;Iqbal M;Legaie R;Gaze WH;Challis GL;Jansen RC;Dijkhuizen L;Rand DA;Wild DL;Bonin M;Reuther J;Wohlleben W;Smith MC;Burroughs NJ;Martín JF;Hodgson DA;Takano E;Breitling R;Ellingsen TE;Wellington EM
通讯作者：
Wellington EM