Dorsal root ganglion neurons promote proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.
Dorsal root ganglion neurons promote proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.
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背根神经节神经元促进骨髓间充质干细胞增殖和成骨分化
DOI：
10.4103/1673-5374.150717
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发表时间：
2015-01
影响因子：
6.1
通讯作者：
Huang F
中科院分区：
文献类型：
--
作者：
Zhang PX;Jiang XR;Wang L;Chen FM;Xu L;Huang F
动物实验初步证实感觉神经纤维促进成骨细胞分化，而运动神经纤维没有促进作用。感觉神经元是否促进骨髓间充质干细胞的增殖和成骨分化尚不清楚。目前还没有细胞水平的结果报道。本研究将体外成骨分化后3周的SD胎鼠背根神经节神经元(感觉神经元)与转绿色荧光蛋白的骨髓间充质干细胞共培养，并以骨髓间充质干细胞来源的成骨细胞作为对照组。共培养3天和5天后，荧光显微镜观察到大鼠背根神经节神经元对骨髓间充质干细胞来源的成骨细胞的增殖有促进作用。实时定量聚合酶链式反应检测，共培养组成骨分化相关因子(碱性磷酸酶、骨钙素、骨桥蛋白、骨形态发生蛋白2)的表达水平高于对照组。结果表明，背根神经节神经元促进了骨髓间充质干细胞的增殖和成骨分化，为构建组织工程骨的体外实验提供了理论依据。
Preliminary animal experiments have confirmed that sensory nerve fibers promote osteoblast differentiation, but motor nerve fibers have no promotion effect. Whether sensory neurons promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells remains unclear. No results at the cellular level have been reported. In this study, dorsal root ganglion neurons (sensory neurons) from Sprague-Dawley fetal rats were co-cultured with bone marrow mesenchymal stem cells transfected with green fluorescent protein 3 weeks after osteogenic differentiation in vitro, while osteoblasts derived from bone marrow mesenchymal stem cells served as the control group. The rat dorsal root ganglion neurons promoted the proliferation of bone marrow mesenchymal stem cell-derived osteoblasts at 3 and 5 days of co-culture, as observed by fluorescence microscopy. The levels of mRNAs for osteogenic differentiation-related factors (including alkaline phosphatase, osteocalcin, osteopontin and bone morphogenetic protein 2) in the co-culture group were higher than those in the control group, as detected by real-time quantitative PCR. Our findings indicate that dorsal root ganglion neurons promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, which provides a theoretical basis for in vitro experiments aimed at constructing tissue-engineered bone.
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影响因子：
2.8
作者：
Li, Jian;Kreicbergs, Andris;Ahmed, Mahmood
通讯作者：
Ahmed, Mahmood
影响因子：
--
作者：
Quartu M;Carozzi VA;Dorsey SG;Serra MP;Poddighe L;Picci C;Boi M;Melis T;Del Fiacco M;Meregalli C;Chiorazzi A;Renn CL;Cavaletti G;Marmiroli P
通讯作者：
Marmiroli P
影响因子：
3
作者：
BJURHOLM, A;KREICBERGS, A;SCHULTZBERG, M
通讯作者：
SCHULTZBERG, M
影响因子：
3.6
作者：
HILL, EL;ELDE, R
通讯作者：
ELDE, R
植入血管束和感觉神经束对体内组织工程骨神经肽受体表达的不同影响
影响因子：
4
作者：
Chen, Si-Yuan;Qin, Jun-Jun;Pei, Guo-Xian
通讯作者：
Pei, Guo-Xian