Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves.
Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves.
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青春期前小牛胚胎发生卵母细胞能力获得的年龄和激素依赖性。
DOI：
10.1095/biolreprod56.2.386
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发表时间：
1997
影响因子：
3.6
通讯作者：
Yang,X
中科院分区：
文献类型：
--
作者：
Presicce,GA;Jiang,S;Simkin,M;Zhang,L;Looney,CR;Godke,RA;Yang,X
本研究以青春期前小母牛为模型，旨在提高我们对卵母细胞成熟和卵母细胞发育能力获得的理解。通过超声引导下经阴道取卵，从20头年龄匹配的5、7、9和11月龄小牛中收集卵母细胞，这些小牛接受或未接受促性腺激素刺激。从未受刺激的小母牛中回收的卵母细胞数量随年龄的增加而减少，分别为15 ± 2、12 ± 1、7 ± 1和7 ± 1（5月龄、7月龄、9月龄和11月龄小牛）。促性腺激素刺激小母牛的相应数量为18 ± 2、16 ± 2、13 ± 1和15 ± 2。卵丘-卵母细胞复合体根据其形态从A到D分级，并且将等级较好的A和B卵母细胞用于体外成熟和受精。在5月龄时，刺激组与未刺激组相比，A级和B级卵母细胞的比例较高（92% vs.49%，p < 0.05）和7月龄（96%对63%，p < 0.05），但刺激对青春期前和青春期后犊牛的这一参数的改善不显著，（53%vs38%，p> 0.05）和11（53%vs38%，p> 0.05）月龄。从5-9月龄未刺激小牛中采集的卵母细胞发育到桑椹胚和囊胚阶段的比例（0- 11%，p < 0.05）低于从年龄匹配但促性腺激素刺激组中采集的卵母细胞（5、7和9月龄分别为10%、39%和31%）。在11月龄的小牛中，有和没有促性腺激素刺激的胚胎发育到桑椹胚和囊胚阶段相似（48%vs.40%，p> 0.05），并且与成年母牛卵母细胞的发育相似（45%，p> 0.05）。这些数据表明，收购的卵母细胞的能力，正常胚胎发育的青春期前小牛的动物年龄和激素治疗的影响。
This research was designed to improve our understanding of oocyte maturation and acquisition of developmental competence of oocytes using prepubertal heifers as a model. Oocytes were collected by ultrasound-guided transvaginal oocyte retrieval from 20 age-matched calves at 5, 7, 9, and 11 mo of age that had or had not received gonadotropin stimulation. Numbers of oocytes recovered from unstimulated heifers decreased with age, being 15 ± 2, 12 ± 1, 7 ± 1, and 7 ± 1 (for 5-, 7-, 9-, and 11-mo-old calves, respectively). Corresponding numbers for the gonadotropin-stimulated heifers were 18 ± 2, 16 ± 2, 13 ± 1, and 15 ± 2. Cumulus-oocyte complexes were graded from A to D on the basis of their morphology, and the better-grade A and B oocytes were used for in vitro maturation and fertilization. A higher proportion of grade A and B oocytes was found for the stimulated vs. unstimulated prepubertal calves at 5 mo of age (92% vs. 49%,p< 0.05) and 7 mo of age (96% vs. 63%,p< 0.05), but the improvement of this parameter by stimulation was not significant for peri- and postpubertal calves at 9 (53% vs. 38%,p> 0.05) and 11 (53% vs. 38%,p> 0.05) mo of age. Embryo development to morula and blastocyst stages was poorer (0–11%,p< 0.05) for oocytes collected from unstimulated calves at 5–9 mo of age than for those from the age-matched but gonadotropin-stimulated groups (10%, 39%, and 31%, at 5, 7, and 9 mo of age, respectively). In calves 11 mo of age, embryo development to morula and blastocyst stages was similar with and without gonadotropin stimulation (48% vs. 40%,p> 0.05) and was comparable to that of adult cow oocytes (45%,p> 0.05). The data suggest that the acquisition of oocyte competence for normal embryo development in prepubertal calves is influenced by animal age and hormonal treatment.
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