Alterations of relaxin and its receptor system components in experimental diabetic cardiomyopathy rats
Alterations of relaxin and its receptor system components in experimental diabetic cardiomyopathy rats
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实验性糖尿病心肌病大鼠松弛素及其受体系统成分的变化
DOI：
10.1007/s00441-017-2662-4
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发表时间：
2017-08
影响因子：
3.6
通讯作者：
Yin Xinhua
中科院分区：
文献类型：
--
作者：
Zhang Xiaohui;Pan Liya;Yang Kelaier;Fu Yu;Liu Yue;Chen Wenjia;Ma Xiao;Yin Xinhua
高糖诱导心肌细胞凋亡和心肌成纤维细胞纤维化，促进糖尿病性心肌病的发生。在这项工作中，我们探讨了实验性糖尿病心肌病大鼠心脏松弛素改变的产生及其受体系统成分的意义。我们测量了实验性糖尿病性心肌病大鼠心脏中大鼠松弛素-1（相当于人松弛素-2）、松弛素-3、RXFP1和RXFP3 mRNA的表达。以5.5 mmol/l正常葡萄糖（NG）和33 mmol/l高糖（HG）分别作用新生大鼠心室肌细胞（nrvm）和心肌成纤维细胞0、6、12、24、48和72 h，采用实时荧光定量PCR检测鼠弛缓素-1、松弛素-3、RXFP1和RXFP3mRNA的表达。在本研究中，我们首次提供了在STZ后4周和8周，糖尿病大鼠心脏中松弛素- 1mrna的表达显著增加，松弛素- 3mrna的表达显著降低的证据。此外，在STZ后4 w， rxfp1和rxfp3的mRNA表达显著下调；然而，在STZ后8周，rxfp1和rxfp3的mRNA表达水平升高。高糖诱导的nrvm凋亡导致松弛素-1和rxfp1水平降低。在hg给药的心脏成纤维细胞中，松弛素- 1mrna显著升高，松弛素- 3mrna显著降低。rxfp1 mRNA表达量降低，rxfp3 mRNA表达量升高。提示松弛素-2、松弛素-3及其受体系统在糖尿病性心肌病的调控中起重要作用。
High glucose induces apoptosis of cardiomyocytes and fibrosis of cardiac fibroblasts, contributing to diabetic cardiomyopathy. In this work, we explore the production of relaxin alterations and the significance of their receptor system components in the hearts of experimental diabetic cardiomyopathy rats. We measured rat relaxin-1 (equivalent to human relaxin-2), relaxin-3, RXFP1 and RXFP3 mRNA expression in the hearts of experimental diabetic cardiomyopathy rats. Neonatal rat ventricular myocytes (NRVMs) and cardiac fibroblasts were treated with 5.5 mmol/l normal glucose (NG) and 33 mmol/l high glucose (HG) for 0, 6, 12, 24, 48 and 72 h. Ratrelaxin-1, relaxin-3, RXFP1 and RXFP3mRNA expression were determined by real-time PCR. In the present study, we offer the first evidence thatRelaxin-1mRNA significantly increased andRelaxin-3mRNA expression decreased at 4 and 8 weeks after STZ in the hearts of diabetic rats. In addition, significant down regulation of the mRNA expression ofRXFP1andRXFP3was observed at 4 w after STZ; however, the mRNA expression levels ofRXFP1andRXFP3were increased at 8 weeks after STZ. Apoptotic NRVMs induced by high glucose generate a decreased level ofrelaxin-1andRXFP1. In HG-administered cardiac fibroblasts,Relaxin-1mRNA was significantly increased andrelaxin-3mRNA was significantly decreased. Additionally, the mRNA expression ofRXFP1was decreased, and the mRNA expression ofRXFP3was increased. This results showed that an important role of relaxin-2, relaxin-3 and their receptors system in the regulation of diabetic cardiomyopathy.
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影响因子：
1.6
作者：
Linda J. Chan;M. Hossain;C. Samuel;F. Separovic;J. Wade
通讯作者：
Linda J. Chan;M. Hossain;C. Samuel;F. Separovic;J. Wade
影响因子：
2.9
作者：
Hossain, Mohammed Akhter;Man, Bryna Chow Suet;Samuel, Chrishan S.
通讯作者：
Samuel, Chrishan S.
影响因子：
3
作者：
Otsubo, Hiroki;Onaka, Tatsushi;Ueta, Yoichi
通讯作者：
Ueta, Yoichi
影响因子：
5.7
作者：
Voors, Adriaan A.;van der Horst, Iwan C. C.
通讯作者：
van der Horst, Iwan C. C.
影响因子：
168.9
作者：
Teerlink, John R.;Metra, Marco;Cotter, Gad
通讯作者：
Cotter, Gad