Pincher-generated Nogo-A endosomes mediate growth cone collapse and retrograde signaling.
Pincher-generated Nogo-A endosomes mediate growth cone collapse and retrograde signaling.
复制标题
DOI：
10.1083/jcb.200906089
复制
发表时间：
2010-01-25
期刊：
影响因子：
--
通讯作者：
Schwab ME
中科院分区：
文献类型：
--
作者：
Joset A;Dodd DA;Halegoua S;Schwab ME
RhoA is activated from internalized Nogo-A to promote growth cone collapse and inhibit neurite outgrowth. Nogo-A is one of the most potent myelin-associated inhibitors for axonal growth, regeneration, and plasticity in the adult central nervous system. The Nogo-A–specific fragment NogoΔ20 induces growth cone collapse, and inhibits neurite outgrowth and cell spreading by activating RhoA. Here, we show that NogoΔ20 is internalized into neuronal cells by a Pincher- and rac-dependent, but clathrin- and dynamin-independent, mechanism. Pincher-mediated macroendocytosis results in the formation of NogoΔ20-containing signalosomes that direct RhoA activation and growth cone collapse. In compartmentalized chamber cultures, NogoΔ20 is endocytosed into neurites and retrogradely transported to the cell bodies of dorsal root ganglion neurons, triggering RhoA activation en route and decreasing phosphorylated cAMP response element binding levels in cell bodies. Thus, Pincher-dependent macroendocytosis leads to the formation of Nogo-A signaling endosomes, which act both within growth cones and after retrograde transport in the cell body to negatively regulate the neuronal growth program.
登录
查看更多内容
抗Nogo-A抗体治疗促进成年灵长类单侧颈椎损伤后手灵巧度的恢复——行为数据的重新检查和扩展。
DOI：
10.1111/j.1460-9568.2009.06642.x
发表时间：
2009-03
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Freund P;Schmidlin E;Wannier T;Bloch J;Mir A;Schwab ME;Rouiller EM
通讯作者：
Rouiller EM
影响因子：
5.3
作者：
Fournier, AE;Takizawa, BT;Strittmatter, SM
通讯作者：
Strittmatter, SM
影响因子：
56.9
作者：
Galko, MJ;Tessier-Lavigne, M
通讯作者：
Tessier-Lavigne, M
影响因子：
16.2
作者：
Gao, Y;Deng, KW;Filbin, MT
通讯作者：
Filbin, MT
DOI：
10.1083/jcb.150.1.145
发表时间：
2000-07-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fish KN;Schmid SL;Damke H
通讯作者：
Damke H