Modulation of Differentiation Processes in Murine Embryonic Stem Cells Exposed to Parabolic Flight-Induced Acute Hypergravity and Microgravity.
Modulation of Differentiation Processes in Murine Embryonic Stem Cells Exposed to Parabolic Flight-Induced Acute Hypergravity and Microgravity.
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暴露于抛物线飞行诱导的急性超高和微重力的鼠类胚胎干细胞中分化过程的调节。
DOI：
10.1089/scd.2017.0294
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发表时间：
2018-06-15
影响因子：
4
通讯作者：
Sachinidis A
中科院分区：
文献类型：
--
作者：
Acharya A;Brungs S;Henry M;Rotshteyn T;Singh Yaduvanshi N;Wegener L;Jentzsch S;Hescheler J;Hemmersbach R;Boeuf H;Sachinidis A
空间微重力条件下的胚胎发育研究非常有限。为了研究短期改变重力对胚胎发育过程的影响，我们将小鼠胚胎干细胞（mESCs）暴露于超重力和微重力阶段，并使用宽基因组微阵列分析研究细胞的分化潜力。在欧洲航天局第64次抛物线飞行活动中，mESC暴露于31个抛物线。每个抛物线包括持续22秒的超重力、微重力和重复的超重力阶段。在不同的抛物线上，分离RNA用于微阵列分析。暴露于31抛物线后，mESC（P31 mESC）在正常重力（1g）条件下进一步分化12天，产生P31 12天胚状体（EB）。微阵列分析后，差异表达的基因进行了分析，使用不同的生物信息学工具，以确定发展和非发展的生物过程中的条件影响的抛物线飞行实验。我们的研究结果表明，一些基因属于与细胞周期和增殖相关的基因在未分化的胚胎干细胞暴露于重力变化下调。然而，属于发育过程（如脉管系统发育、肾脏发育、皮肤发育）和TGF-β信号传导途径的几个基因被上调。有趣的是，与地面对照12天的EB相比，在P31 12天的EB中获得了类似的富集和抑制的ESTA。我们的研究结果表明，暴露于交替超重力和微重力阶段的未分化的胚胎干细胞表达与发育/分化和细胞周期过程相关的几个基因，这表明从未分化的多能过渡到一个更分化的阶段的胚胎干细胞。
Embryonic developmental studies under microgravity conditions in space are very limited. To study the effects of short-term altered gravity on embryonic development processes, we exposed mouse embryonic stem cells (mESCs) to phases of hypergravity and microgravity and studied the differentiation potential of the cells using wide-genome microarray analysis. During the 64th European Space Agency's parabolic flight campaign, mESCs were exposed to 31 parabolas. Each parabola comprised phases lasting 22 s of hypergravity, microgravity, and a repeat of hypergravity. On different parabolas, RNA was isolated for microarray analysis. After exposure to 31 parabolas, mESCs (P31 mESCs) were further differentiated under normal gravity (1 g) conditions for 12 days, producing P31 12-day embryoid bodies (EBs). After analysis of the microarrays, the differentially expressed genes were analyzed using different bioinformatic tools to identify developmental and nondevelopmental biological processes affected by conditions on the parabolic flight experiment. Our results demonstrated that several genes belonging to GOs associated with cell cycle and proliferation were downregulated in undifferentiated mESCs exposed to gravity changes. However, several genes belonging to developmental processes, such as vasculature development, kidney development, skin development, and to the TGF-β signaling pathway, were upregulated. Interestingly, similar enriched and suppressed GOs were obtained in P31 12-day EBs compared with ground control 12-day EBs. Our results show that undifferentiated mESCs exposed to alternate hypergravity and microgravity phases expressed several genes associated with developmental/differentiation and cell cycle processes, suggesting a transition from the undifferentiated pluripotent to a more differentiated stage of mESCs.
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影响因子：
--
作者：
Maier JA;Cialdai F;Monici M;Morbidelli L
通讯作者：
Morbidelli L
影响因子：
5.1
作者：
Hauslage J;Cevik V;Hemmersbach R
通讯作者：
Hemmersbach R
影响因子：
2.6
作者：
Plett, PA;Abonour, R;Orschell, CM
通讯作者：
Orschell, CM
影响因子：
8.5
作者：
Dai, Z. Q.;Wang, R.;Li, Y. H.
通讯作者：
Li, Y. H.
影响因子：
4
作者：
Monticone, Massimiliano;Liu, Yi;Cancedda, Ranieri
通讯作者：
Cancedda, Ranieri