Cytotoxicity and Effects on the Synapsis Induced by Pure Cylindrospermopsin in an E17 Embryonic Murine Primary Neuronal Culture in a Concentration- and Time-Dependent Manner.
Cytotoxicity and Effects on the Synapsis Induced by Pure Cylindrospermopsin in an E17 Embryonic Murine Primary Neuronal Culture in a Concentration- and Time-Dependent Manner.
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DOI：
10.3390/toxins14030175
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发表时间：
2022-02-26
期刊：
影响因子：
4.2
通讯作者：
Jos Á
中科院分区：
文献类型：
--
作者：
Hinojosa MG;Prieto AI;Muñoz-Castro C;Sánchez-Mico MV;Vitorica J;Cameán AM;Jos Á
草履虫毒素（CYN）是一种在过去几十年中发病率不断上升的蓝藻毒素。由于它能够在生物体的不同水平上造成损害，因此被认为是一种细胞毒素。虽然主要靶器官是肝脏，但最近的研究表明，CYN在体外和体内对神经系统都有潜在的毒性作用。因此，本工作的目的是研究这种氰毒素对暴露于环境相关浓度（0-1 µg/mL CYN）12、24和48 h的小鼠神经元原代培养物中神经元活力和突触完整性的影响。结果表明细胞活力呈浓度和时间依赖性降低;暴露于氰毒素12 h后未检测到细胞毒性，而所有测定浓度均在48 h后降低了该参数。此外，CYN还被证明在小鼠原代神经元培养物中以浓度和时间依赖性方式在突触水平上产生损伤。这些数据突出了在不同的实验模型中研究这种氰毒素的神经毒性特性的重要性。
Cylindrospermopsin (CYN) is a cyanotoxin whose incidence has been increasing in the last decades. Due to its capacity to exert damage at different levels of the organism, it is considered a cytotoxin. Although the main target organ is the liver, recent studies indicate that CYN has potential toxic effects on the nervous system, both in vitro and in vivo. Thus, the aim of the present work was to study the effects of this cyanotoxin on neuronal viability and synaptic integrity in murine primary cultures of neurons exposed to environmentally relevant concentrations (0–1 µg/mL CYN) for 12, 24, and 48 h. The results demonstrate a concentration- and time-dependent decrease in cell viability; no cytotoxicity was detected after exposure to the cyanotoxin for 12 h, while all of the concentrations assayed decreased this parameter after 48 h. Furthermore, CYN was also demonstrated to exert damage at the synaptic level in a murine primary neuronal culture in a concentration- and time-dependent manner. These data highlight the importance of studying the neurotoxic properties of this cyanotoxin in different experimental models.
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影响因子：
4.1
作者：
Cossette, M;Lévesque, D;Parent, A
通讯作者：
Parent, A
影响因子：
4.5
作者：
Guzman-Guillen, Remedios;Lomares Manzano, Inmaculada;Camean, Ana M.
通讯作者：
Camean, Ana M.
影响因子：
5.5
作者：
Béïque, JC;Andrade, R
通讯作者：
Andrade, R
DOI：
10.1080/15287394.2018.1460787
发表时间：
2018-01-01
影响因子：
2.6
作者：
Chernoff, N.;Hilla, D. J.;Wood, C. R.
通讯作者：
Wood, C. R.
影响因子：
6.1
作者：
Guzman-Guillen, Remedios;Prieto Ortega, Ana I.;Camean, Ana M.
通讯作者：
Camean, Ana M.