Magnetite accelerates syntrophic acetate oxidation in methanogenic systems with high ammonia concentrations.
Magnetite accelerates syntrophic acetate oxidation in methanogenic systems with high ammonia concentrations.
复制标题
磁铁矿加速氨浓度高的产甲烷系统中的互养乙酸盐氧化
DOI：
10.1111/1751-7915.13286
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发表时间：
2018-07
影响因子：
5.7
通讯作者：
Tang J
中科院分区：
文献类型：
--
作者：
Zhuang L;Ma J;Yu Z;Wang Y;Tang J
氨积累是导致厌氧消化紊乱和 CH4 生产失败的主要抑制物质。在高氨水平下，通过自养乙酸氧化（SAO）途径生产CH4比乙酰分解产甲烷途径更能耐受氨毒性，但通过SAO的低CH4产率是处理富氨底物的厌氧消化池能量回收效率低的主要原因。在这项研究中，我们发现，在含有高氨浓度（5.0 g l−1 NH4+–N）的厌氧反应器中，乙酸盐发酵成CH4和CO2是通过SAO途径发生的，并且与相同氨水平下不含磁铁矿的厌氧反应器相比，添加磁铁矿纳米颗粒使乙酸盐中CH4的产率提高了36-58%。促进产甲烷的机制被认为是SAO建立直接种间电子转移（DIET），其中磁铁矿促进了互养乙酸盐氧化细菌和产甲烷菌之间的DIET。高通量 16S rRNA 基因测序分析表明，细菌 Geobacteraceae 和古菌 Methanosarcinaceae 和 Methanobacteriaceae 可能参与磁铁矿介导的 DIET 生产 SAO 和 CH4。这项研究表明，补充磁铁矿可能提供一种有效的方法来加速处理高氨废水的厌氧反应器中的 CH4 生产率。
Ammonia accumulation is a major inhibitory substance causing anaerobic digestion upset and failure in CH4 production. At high ammonia levels, CH4 production through syntrophic acetate oxidization (SAO) pathways is more tolerant to ammonia toxicity than the acetoclastic methanogenesis pathway, but the low CH4 production rate through SAO constitutes the main reason for the low efficiency of energy recovery in anaerobic digesters treating ammonia‐rich substrates. In this study, we showed that acetate fermentation to CH4 and CO2 occurred through SAO pathway in the anaerobic reactors containing a high ammonia concentration (5.0 g l−1 NH4+–N), and the magnetite nanoparticles supplementation increased the CH4 production rates from acetate by 36–58%, compared with the anaerobic reactors without magnetite under the same ammonia level. The mechanism of facilitated methanogenesis was proposed to be the establishment of direct interspecies electron transfer (DIET) for SAO, in which magnetite facilitated DIET between syntrophic acetate oxidizing bacteria and methanogens. High‐throughput 16S rRNA gene sequencing analysis revealed that the bacterial Geobacteraceae and the archaeal Methanosarcinaceae and Methanobacteriaceae might be involved in magnetite‐mediated DIET for SAO and CH4 production. This study demonstrated that magnetite supplementation might provide an effective approach to accelerate CH4 production rates in the anaerobic reactors treating wastewater containing high ammonia.
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影响因子：
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通讯作者：
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