Artificially acquired chlorophyll b is highly acceptable to the thylakoid-lacking cyanobacterium, Gloeobacter violaceus PCC 7421.
Artificially acquired chlorophyll b is highly acceptable to the thylakoid-lacking cyanobacterium, Gloeobacter violaceus PCC 7421.
复制标题
人工获得的叶绿素 b 对于缺乏类囊体的蓝细菌(Gloeobacter violaceus PCC 7421)来说是高度可接受的。
DOI:
10.1016/j.plaphy.2014.01.006
复制
发表时间:
2014
期刊:
影响因子:
--
通讯作者:
T.
中科院分区:
文献类型:
--
作者:
Araki;M.;Akimoto;S.;Mimuro;M.;Tsuchiya;T.
Unicellular cyanobacterium Gloeobacter violaceus is an only known oxygenic photosynthetic organism that lacks thylakoid membrane. Molecular phylogenetic analyses indicate thatG. violaceusis an early-branching cyanobacterium within cyanobacterial clade. Therefore, the photosynthetic system ofG. violaceusis considered to be partly similar to that of the ancestral cyanobacteria that would lack thylakoid membrane.G. violaceuspossesses chlorophyll (Chl)aas the only chlorophyll species like most cyanobacteria. It was proposed that the ancestral oxygenic photosynthetic organism had not only Chlaand phycobilins but also Chlb. However, no organism which contains both Chlaand Chlband lacks thylakoid membrane has been found in nature. Therefore, we introduced the chlorophyllideaoxygenase gene responsible for Chlbbiosynthesis intoG. violaceus. In the resultant transformant, Chlbaccumulated at approximately 11% of total Chl independent of growth phase. Photosystem I complexes isolated from the transformant contained Chlbat 9.9% of total Chl. The presence of Chlbin the photosystem I complexes did not inhibit trimer formation. Furthermore, time-resolved fluorescence spectrum demonstrated that Chlbtransferred energy to Chlain the photosystem I complexes and did not disturb the energy transfer among the Chlamolecules. These results show thatG. violaceusis tolerant to artificially produced Chlband suggest the flexibility of photosystem for Chl composition in the ancestral oxygenic photosynthetic organism.
登录
查看更多内容
DOI:
--
发表时间:
1998
期刊:
影响因子:
--
作者:
C. Jansson;G. Salih;J. Eriksson;Ronney Wiklund;Haile Ghebramedhin
通讯作者:
Haile Ghebramedhin
DOI:
10.1016/j.bbabio.2006.01.006
发表时间:
2006
期刊:
Biochimica et biophysica acta
影响因子:
--
作者:
E. Gutiérrez;B. Pérez;D. Krogmann;C. Gómez
通讯作者:
C. Gómez
DOI:
--
发表时间:
--
期刊:
影响因子:
--
作者:
通讯作者:
--
DOI:
--
发表时间:
2003
期刊:
影响因子:
--
作者:
H. Miyashita;H. Ikemoto;N. Kurano;S. Miyachi;M. Chihara
通讯作者:
M. Chihara
DOI:
--
发表时间:
2004
期刊:
FEBS Letters 578
影响因子:
--
作者:
Inoue;H.;他7名(8番目)
通讯作者:
他7名(8番目)