The phycocyanin-associated rod linker proteins of the phycobilisome of Gloeobacter violaceus PCC 7421 contain unusually located rod-capping domains.
The phycocyanin-associated rod linker proteins of the phycobilisome of Gloeobacter violaceus PCC 7421 contain unusually located rod-capping domains.
复制标题
紫色球杆菌 PCC 7421 藻胆体的藻蓝蛋白相关杆接头蛋白含有异常定位的杆帽结构域。
DOI：
10.1016/j.bbabio.2006.01.006
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
C. Gómez
中科院分区：
文献类型：
--
作者：
E. Gutiérrez;B. Pérez;D. Krogmann;C. Gómez
紫球藻PCC 7421是一种独特的无类囊体蓝藻，其基因组已被测序[Y.Nakamura，T.Kaneko，S.Sato，M.Mimuro，H.Miyashita，T.T.Tsuhiya，S.Sasamoto，A.Watanabe，K.Kawashima，Y.Kishida，C.Kiyokawa，M.Kohara，M.MatSumoto，A.Matsuno，N.Nakazaki，S.Shimpo，C.Takeuchi，M.Yamada，S.Tabata，完整基因组结构Gloebacter Viaceus PCC 7421，一个缺乏类囊体体的蓝藻。DNA Research 10(2003)137-145]。分离和分析了诸葛菜的藻胆体，并对其进行了N末端测序。根据基因组序列确定了3个杆状连接亚基(CPEC、CpeD和CpeE)。编码紫罗兰藻蓝蛋白相关连接蛋白的(OLN)glr0950和gll 3219上的cpcC1和cpcC2基因在N端分别比基因组中提出的开放阅读框长56和55个氨基酸。这两个氨基酸延伸部分显示出66%的同源性。此外，这些序列的N-末端延伸与杆帽连接蛋白CpcD2中的结构域和藻红蛋白相关连接蛋白CPEC的C-末端结构域相似。这些结构域不仅在N端位置上不寻常，而且因为它们在序列上与藻红蛋白相关连接物CPEC的C末端结构域的相似性比与其他蓝藻中藻蓝蛋白相关连接物CPCC的C末端结构域更密切。这些具有独特结构域的连接蛋白是紫罗兰藻胆体特殊结构的指示物。
Gloeobacter violaceus PCC 7421 is a unique cyanobacterium that has no thylakoids and whose genome has been sequenced [Y. Nakamura, T. Kaneko, S. Sato, M. Mimuro, H. Miyashita, T. Tsuchiya, S. Sasamoto, A. Watanabe, K. Kawashima, Y. Kishida, C. Kiyokawa, M. Kohara, M. Matsumoto, A. Matsuno, N. Nakazaki, S. Shimpo, C. Takeuchi, M. Yamada, S. Tabata, Complete Genome Structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids. DNA Research 10 (2003) 137–145]. Phycobilisomes of G. violaceus were isolated and analyzed by SDS-PAGE followed by N-terminal sequencing. Three rod-linker subunits (CpeC, CpeD and CpeE) were identified as predicted from the genome sequence. The cpcC1 and cpcC2 genes at order locus named (OLN) glr0950 and gll 3219 encoding phycocyanin-associated linker proteins from G. violaceus are 56 and 55 amino acids longer at the N-terminus than the open reading frame proposed in the genome. The two amino acid extensions showed a 66% identity to one another. Also, the N-terminal extensions of these sequences were similar to domains in both the rod-capping-linker protein CpcD2 and to the C-terminus domain of the phycoerythrin-associated linker protein CpeC. These domains are not only unusual in their N-terminal location, but are unusual in that they are more closely related in sequence similarity to the C-terminus domain of the phycoerythrin-associated linker, CpeC of G. violaceus, than to the C-terminus domain of phycocyanin-associated linker CpcC in other cyanobacteria. These linker proteins with unique special domains are indicators of the unusual structure of the phycobilisomes of G. violaceus.
影响因子：
3.5
作者：
BRYANT, DA;STIREWALT, VL;ZUBER, H
通讯作者：
ZUBER, H
影响因子：
2.9
作者：
Gómez-Lojero, C;Pérez-Gómez, B;Bryant, DA
通讯作者：
Bryant, DA