Effect of 3D-Fibroblast Dermis Constructed by Layer-by-Layer Cell Coating Technique on Tight Junction Formation and Function in Full-Thickness Skin Equivalent.
Effect of 3D-Fibroblast Dermis Constructed by Layer-by-Layer Cell Coating Technique on Tight Junction Formation and Function in Full-Thickness Skin Equivalent.
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逐层细胞涂层技术构建的 3D 成纤维细胞真皮对全层皮肤等效物紧密连接形成和功能的影响。
DOI：
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发表时间：
2021
影响因子：
5.8
通讯作者：
M. Akashi
中科院分区：
文献类型：
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作者：
Masato Murakami;T. Akagi;Yumi Sasano;M. Akashi
由表皮和真皮组成的人体皮肤替代物(HSE)已被用作药物评价和再生医学临床应用的有前景的工具。正常人真皮成纤维细胞(NHDF)在表皮成熟过程中起着重要作用，因此对HSE的构建是必不可少的。近年来，作为第二屏障和其他屏障功能调节因子的表皮紧密连接(TJ)，作为一种复杂的细胞-细胞连接，受到了广泛的关注。在之前的研究中，我们揭示了用逐层(LBL)细胞涂层技术构建的HSE(Layer-by-Layer-3D)皮肤中TJ相关蛋白的表达和TJ结构形成的时间进程，该皮肤具有仅由NHDF组成的独特真皮(3D-成纤维细胞真皮)。然而，3D成纤维细胞真皮对功能性表皮TJ形成的影响尚不清楚。在本研究中，我们研究了3D成纤维细胞真皮对LBL-3D皮肤TJ相关蛋白表达和TJ功能的影响。我们证明3D-成纤维细胞真皮影响TJ相关蛋白的长期表达，并影响具有屏障功能的TJ在表皮中的形成。这些结果表明，LBL-3D皮肤中的3D-成纤维细胞真皮通过与KCs的直接接触，促进了功能性TJ的形成和维持，就像在自然皮肤中一样。
Human skin equivalents (HSEs) consisting of an epidermis and dermis have been used as promising tools for drug evaluation and for clinical applications in regenerative medicine. Normal human dermal fibroblasts (NHDFs) are essential for the fabrication of HSEs because they play an important role in the maturation of the epidermis. Recently, epidermal tight junctions (TJs), which are complex cell-cell junctions, have attracted much attention as a second barrier and regulator for other barrier functions. In a previous study, we revealed the expression of TJ-related proteins and the time course of formation of TJ structure in the HSE (layer-by-layer (LbL)-three-dimensional (3D) Skin) constructed by layer-by-layer (LbL) cell coating technique that have a unique dermis consisting of NHDFs only (3D-fibroblast dermis). However, the effect of the 3D-fibroblast dermis on the formation of functional epidermal TJs is unknown. In this study, we investigated the effect of the 3D-fibroblast dermis on the expression of TJ-related proteins and TJ function in LbL-3D Skin. We demonstrated that the 3D-fibroblast dermis affects the long-term expression of TJ-related proteins and the formation of TJ with barrier function in the epidermis. These results show that the 3D-fibroblast dermis in LbL-3D Skin contributes to the formation and maintenance of functional TJs as in native human skin by direct contact with KCs.
DOI：
10.1111/j.1749-6632.2012.06554.x
发表时间：
2012-01-01
期刊：
BARRIERS AND CHANNELS FORMED BY TIGHT JUNCTION PROTEINS I
影响因子：
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作者：
Kirschner, Nina;Brandner, Johanna M.
通讯作者：
Brandner, Johanna M.