31P-nuclear magnetic resonance analysis of the intracellular pH in the slime mold Dictyostelium discoideum.
31P-nuclear magnetic resonance analysis of the intracellular pH in the slime mold Dictyostelium discoideum.
复制标题
粘菌盘基网柄菌细胞内pH值的31P-核磁共振分析。
DOI：
10.1016/0006-291x(85)90999-4
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
J. B. Martin
中科院分区：
文献类型：
--
作者：
M. Satre;J. B. Martin
Amoebae of the slime moldD. discoideumwere studied by phosphorus nuclear magnetic resonance. Under aerobic conditions, major intracellular phosphate compounds included phosphomonoesters, inorganic phosphate (Pi), ADP and ATP. Nucleotides were essentially as magnesium complexes. Two intracellular Pi resonances were clearly resolved and the corresponding pHs determined by the chemical shifts characteristics were 7.7 and 6.7. These intracellular pHs were strictly constant over an extracellular pH range between 5.0 and 7.5. The two cellular compartments defined by the Pi resonances were assigned to mitochondria (pH 7.7) and cytosol (pH 6.7) on the basis of their response to anaerobiosis or to carbonylcyanide-m-chloro phenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, which equilibrate the two intracellular pHs.
细胞内磷酸盐库显示龟膀胱中细胞内 pH 值的划分。
DOI：
10.1016/0006-291x(81)90682-3
发表时间：
1981
影响因子：
3.1
作者：
Arruda,JA;Lubansky,H;Sabatini,S;Dytko,G;Burt,CT
通讯作者：
Burt,CT