Oral microbial biofilm stimulation of epithelial cell responses.
Oral microbial biofilm stimulation of epithelial cell responses.
复制标题
口服微生物生物膜刺激上皮细胞反应。
DOI：
10.1016/j.cyto.2011.12.016
复制
发表时间：
2012-04
期刊：
影响因子：
3.8
通讯作者：
Ebersole, Jeffrey L.
中科院分区：
文献类型：
--
作者：
Peyyala, Rebecca;Kirakodu, Sreenatha S.;Novak, Karen F.;Ebersole, Jeffrey L.
口腔细菌生物膜引发宿主的慢性炎症反应，可导致牙周炎的组织破坏事件。然而，特定宿主细胞类型对这些生物膜的反应能力的特征仍然不明确。本报告描述了使用一种新的细菌生物膜模型来刺激口腔上皮细胞，并描述了在牙周组织中促进局部炎症环境的细胞因子和趋化因子的选择。在硬质透气性隐形眼镜上形成血链球菌、口腔链球菌、戈多氏链球菌、纳氏放线菌、核梭菌和牙龈卟啉单胞菌的单感染生物膜。这些细菌的生物膜和浮游培养物在厌氧条件下与人类口腔上皮细胞系OKF4一起培养24小时。Gro-1α、il -1α、IL-6、IL-8、TGFα、Fractalkine、MIP-1α和IP-10被证明是对这些细菌的一系列浮游或生物膜形式的反应。除MIP-1α外，牙龈假单胞菌生物膜显著抑制所有这些细胞因子和趋化因子的产生。总的来说，所有物种的生物膜都抑制了Gro-1α、TGFα和Fractalkine的产生，而F. nucleatum生物膜则刺激了IL-1α、IL-6、IL-8和IP-10的显著增加。naeslundii生物膜诱导IL-6、IL-8和IP-10水平升高。生物膜或浮游形式的口腔链球菌对来自上皮细胞的任何这些介质的刺激性都很差。这些研究的结果表明，口腔生物膜中的细菌与同一物种的浮游细菌相比，引起了本质上不同的反应。此外，某些口腔细菌在生物膜中具有高度刺激性，并与宿主细胞受体相互作用，从而触发与个体细菌截然不同的反应途径。
Oral bacterial biofilms trigger chronic inflammatory responses in the host that can result in the tissue destructive events of periodontitis. However, the characteristics of the capacity of specific host cell types to respond to these biofilms remain ill-defined. This report describes the use of a novel model of bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. Monoinfection biofilms were developed with Streptococcus sanguinis, Streptococcus oralis, Streptococcus gordonii, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis on rigid gas-permeable contact lenses. Biofilms, as well as planktonic cultures of these same bacterial species, were incubated under anaerobic conditions with a human oral epithelial cell line, OKF4, for up to 24 h. Gro-1α, IL1α, IL-6, IL-8, TGFα, Fractalkine, MIP-1α, and IP-10 were shown to be produced in response to a range of the planktonic or biofilm forms of these species. P. gingivalis biofilms significantly inhibited the production of all of these cytokines and chemokines, except MIP-1α. Generally, the biofilms of all species inhibited Gro-1α, TGFα, and Fractalkine production, while F. nucleatum biofilms stimulated significant increases in IL-1α, IL-6, IL-8, and IP-10. A. naeslundii biofilms induced elevated levels of IL-6, IL-8 and IP-10. The oral streptococcal species in biofilms or planktonic forms were poor stimulants for any of these mediators from the epithelial cells. The results of these studies demonstrate that oral bacteria in biofilms elicit a substantially different profile of responses compared to planktonic bacteria of the same species. Moreover, certain oral species are highly stimulatory when in biofilms and interact with host cell receptors to trigger pathways of responses that appear quite divergent from individual bacteria.
登录
查看更多内容
影响因子：
3.8
作者：
Belibasakis, Georgios N.;Meier, Andre;Bostanci, Nagihan
通讯作者：
Bostanci, Nagihan
影响因子：
14.9
作者：
Kolenbrander PE
通讯作者：
Kolenbrander PE
DOI：
10.1099/13500872-140-12-3407
发表时间：
1994-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
BRADSHAW, DJ;HOMER, KA;BEIGHTON, D
通讯作者：
BEIGHTON, D
影响因子：
4.2
作者：
Guggenheim, Bernhard;Gmuer, Rudolf;Kinane, Denis F.
通讯作者：
Kinane, Denis F.
影响因子：
3.1
作者：
Huang, GTJ;Kim, D;Haake, SK
通讯作者：
Haake, SK