Transport of organic anions and cations in isolated renal plasma membranes.
Transport of organic anions and cations in isolated renal plasma membranes.
复制标题
有机阴离子和阳离子在离体肾质膜中的转运。
DOI：
--
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发表时间：
1983
影响因子：
12.5
通讯作者：
P. Holohan
中科院分区：
文献类型：
--
作者：
C. Ross;P. Holohan
在近端肾小管细胞中，有机阴离子和阳离子（以前称为酸和碱）的经上皮运输已经得到了很好的记录。后一种命名法是不正确的，因为从严格意义上讲，含季氮化合物不是化学碱。有机离子的运动顺序是通过基底外侧膜运输，在细胞内积聚，然后从细胞流出，穿过刷状边界进入管状流体（见1-6）。证明这些现象的技术有很多，Torretti和Weiner(7)和Rennick(8)在综述中概述了这些技术。它们包括肾清除率、Sperber技术、停流、逆行静脉注射和微穿刺等体内技术。在肾切片和离体肾小管中研究了体外转运。总的来说，这些技术已经确定了这两个系统是分开的和不同的，两组化合物都是通过“载体”介导的运输积极分泌的。阴离子和阳离子都能够“上坡”运输（逆浓度梯度运动）。每个系统都是饱和的，可被代谢抑制剂抑制，显示同类化合物之间的竞争，并显示双向运输。虽然所有这些发现都产生了大量关于整个事件序列的信息，但它们未能产生关于刷状边界和基底膜对整个上皮现象的相对贡献的具体信息。因此，本综述强调所作的贡献以及提出的问题
The transepithelial transport in proximal renal tubular cells has been well documented for both organic anions and cations, previously referred to as acids and bases. The latter nomenclature is incorrect as quaternary nitro gen-containing compounds are not chemical bases in the strictest sense. The sequence of movement of organic ions is transport across the basolateral membrane, accumulation in the cells, followed by efflux from the cell across the brush border into the tubular fluid (see 1-6), The techniques for demonstrating these phenomena are numerous and have been outlined in reviews by Torretti & Weiner (7) and Rennick (8). They include such in vivo techniques as renal clearance, Sperber technique, stop-flow, retrograde intraureteral injection, and micropuncture. In vitro transport has been studied in renal slices and isolated renal tubules. Collec tively these techniques have established that the two systems are separate and distinct and that both groups of compounds are actively secreted by "carrier"-mediated transport. Both anions and cations are capable of being transported "uphill" (movement against a concentration gradient). Each system is saturable, inhibitable by metabolic inhibitors, demonstrates com petition between compounds within the same class, and demonstrates bidi rectional transport. While all of these findings have generated a great deal of information about the overall sequence of events, they have failed to yield specific information on the relative contributions of the brush border and basolat eral membranes to the overall transepithelial phenomena. Therefore, this review emphasizes the contributions made as well as the questions raised
尿酸盐的肾脏排泄：决定药物作用的因素。
影响因子：
19.6
作者：
Roch-Ramel,F;Weiner,IM
通讯作者：
Weiner,IM
一种从大鼠肾脏中分离基底外侧膜的改进方法。
DOI：
10.1016/0005-2736(80)90516-7
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Reynolds,RA;Wald,H;McNamara,PD;Segal,S
通讯作者：
Segal,S
肾刷状缘膜中琥珀酸钠协同转运的化学计量。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wright,SH;Kippen,I;Wright,EM
通讯作者：
Wright,EM