Transcriptome analysis of wheat grain using RNA-Seq
Transcriptome analysis of wheat grain using RNA-Seq
复制标题
使用 RNA-Seq 对小麦籽粒进行转录组分析
DOI：
10.15302/j-fase-2014024
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Baoyun Li
中科院分区：
文献类型：
--
作者：
Liu Wei;Zhihui Wu;Yufeng Zhang;D;an Guo;Yuzhou Xu;Weixia Chen;Haiying Zhou;Mingshan You;Baoyun Li
随着消费需求的增加，小麦品质改良已成为中国乃至世界关注的焦点。转录组分析是研究谷物性状和阐明其遗传调控的一种强有力的方法。本研究利用Roche/454技术对面包小麦品种农大211的籽粒和叶茎发育过程中的两个cDNA文库进行了测序。籽粒和叶茎产生的清洁读数分别为1061274和1516564。经重新组装后，共获得61393个高质量的非基因区，平均长度为1456bp。对参与籽粒生物过程的61393个非基因的分析表明，有7355个差异表达基因在籽粒文库中上调。基因本体论丰富和京都基因和基因组途径丰富分析表明，许多与碳水化合物和蛋白质代谢相关的转录产物和转录因子在籽粒中得到了丰富的表达。这些结果有助于挖掘与小麦品质相关的类别，并进一步研究它们之间的相互作用。
With the increase in consumer demand, wheat.grain quality improvement has become a focus in China.and worldwide. Transcriptome analysis is a powerful.approach to research grain traits and elucidate their genetic.regulation. In this study, two cDNA libraries from the.developing grain and leaf-stem components of bread wheat.cultivar, Nongda211, were sequenced using Roche/454.technology. There were 1061274 and 1516564 clean reads.generated from grain and leaf-stem, respectively. A total of.61393 high-quality unigenes were obtained with an.average length of 1456 bp after de novo assembly. The.analysis of the 61393 unigenes involved in the biological.processes of the grain showed that there were 7355.differentially expressed genes upregulated in the grain.library. Gene ontology enrichment and the Kyoto Ency-.clopedia of Genes and Genomes pathway enrichment.analysis showed that many transcription products and.transcription factors associated with carbohydrate and.protein metabolism were abundantly expressed in the.grain. These results contribute to excavategenes associated.with wheat quality and further study how they interact.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
5.1
作者：
Dong, Guoqing;Ni, Zhongfu;Sun, Qixin
通讯作者：
Sun, Qixin
DOI：
10.1046/j.1432-1327.2000.01199.x
发表时间：
2000-04-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Franco, OL;Rigden, DJ;de Sá, MFG
通讯作者：
de Sá, MFG
影响因子：
3.7
作者：
Xu H;Gao Y;Wang J
通讯作者：
Wang J
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Stefan Go¨tz;Juan Miguel Garcı´a-Go´mez;Javier Terol;Tim D. Williams;Shivashankar H. Nagaraj;Marı´a
通讯作者：
Stefan Go¨tz;Juan Miguel Garcı´a-Go´mez;Javier Terol;Tim D. Williams;Shivashankar H. Nagaraj;Marı´a