Comparative interaction of ?-helical and ?-sheet amphiphilic isopeptides with phospholipid monolayers
Comparative interaction of ?-helical and ?-sheet amphiphilic isopeptides with phospholipid monolayers
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β-螺旋和β-片层两亲异肽与磷脂单层的相互作用比较
DOI：
10.1002/1097-0282(200107)59:1
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发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
D. Lelièvre
中科院分区：
文献类型：
--
作者：
R. Maget;D. Lelièvre
两种连续的两亲性肽异构体（Leu-Lys-Lys-Leu）4 和（Leu-Lys）8 分别被选为α螺旋和β折叠肽的模型。为了评估肽的二级结构对其渗透到细胞膜的贡献，研究了这些异肽与 L-α-二肉豆蔻酰磷脂酰胆碱 (DMPC) 单层的相互作用。两种异肽都能以约 27 mN/m 的排阻压力渗入 DMPC 单层，但在 α 螺旋 (LKKL)4 的渗透曲线中观察到不连续性。分析了从混合肽/DMPC单层的压缩等温线中提取的主要参数，即转变压力、平均面积、弹性模量和混合能量。这些分析表明，α-螺旋异构体通过形成 1:32 (LKKL)4-DMPC 复合物与 DMPC 发生强烈相互作用。当参与该复合物时，(LKKL)(4) 表现为疏水性螺旋，并且在压缩过程中倾向于变为垂直方向。 β-片层 (LK)8 与 DMPC 的相互作用较弱，未检测到复合物。
The two sequential amphiphilic peptide isomers, (Leu-Lys-Lys-Leu)4 and (Leu-Lys)8, were chosen as models for alpha-helical and beta-sheet peptides, respectively. In order to evaluate the contribution of the secondary structure of a peptide to its penetration into cellular membranes, interactions of these isopeptides with L-alpha-dimyristoyl phosphatidylcholine (DMPC) monolayers were studied. Both isopeptides penetrate into DMPC monolayers up to an exclusion pressure of approximately 27 mN/m, but a discontinuity is observed in the penetration profile of the alpha-helical (LKKL)4. The main parameters extracted from the compression isotherms of the mixed peptide/DMPC monolayers-namely, transition pressure, mean area, elasticity modulus, and energy of mixing-were analyzed. These analyses indicate that the alpha-helical isomer interacts strongly with DMPC by forming a 1:32 (LKKL)4-DMPC complex. When engaged in this complex, (LKKL)(4) behaves as an hydrophobic helix and has a tendency to become vertically oriented in the course of the compression process. The beta-sheet (LK)8 interacts more weakly with DMPC and no complex can be detected.
影响因子：
3.4
作者：
DEGRADO, WF;MUSSO, GF;KEZDY, FJ
通讯作者：
KEZDY, FJ
乙型肝炎病毒与磷脂相互作用后推定的融合肽的结构特性。
DOI：
10.1111/j.1432-1033.1996.0243r.x
发表时间：
1996
期刊：
European journal of biochemistry
影响因子：
--
作者：
Rodríguez-Crespo,I;Gómez-Gutiérrez,J;Encinar,JA;González-Ros,JM;Albar,JP;Peterson,DL;Gavilanes,F
通讯作者：
Gavilanes,F