Vaccination with Neospora GRA6 Interrupts the Vertical Transmission and Partially Protects Dams and Offspring against Neospora caninum Infection in Mice.
Vaccination with Neospora GRA6 Interrupts the Vertical Transmission and Partially Protects Dams and Offspring against Neospora caninum Infection in Mice.
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DOI：
10.3390/vaccines9020155
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发表时间：
2021-02-15
期刊：
影响因子：
7.8
通讯作者：
Nishikawa Y
中科院分区：
文献类型：
--
作者：
Fereig RM;Abdelbaky HH;Nishikawa Y
疫苗接种是许多传染病预防措施的支柱。犬新孢子虫感染会引发怀孕母牛和母羊的流产风暴，由于胎儿死亡和母羊被扑杀而造成巨大的经济损失。在此，我们评估了与谷胱甘肽-S-转移酶融合的犬新孢子虫致密颗粒蛋白 6 的重组蛋白 (NcGRA6+GST) 作为妊娠小鼠模型中新孢子虫病候选疫苗的潜力。通过给 BALB/c 小鼠皮下接种重组 NcGRA6+GST (25 pmol)、单独接种 GST (25 pmol) 或磷酸盐缓冲盐水 (PBS) 作为对照，研究其保护效果。本研究揭示了 NcGRA6+GST 在妊娠关键期部分保护母鼠和后代免受犬新孢子虫感染的能力。与对照组相比，NcGRA6+GST 免疫组的后代大脑中的存活率更高，寄生虫负荷更低，揭示了这种能力。此外，与 PBS 或 GST 组相比，NcGRA6+GST 免疫组的小鼠在用犬新孢子虫速殖子攻击后表现出较低的临床评分和最小的体重变化。综上所述，我们的结果表明重组 NcGRA6 通过降低母鼠和后代感染的严重程度来阻断小鼠中犬新孢子虫的垂直传播。
Vaccination is the mainstay of preventative measures for numerous infectious diseases. Neospora caninum infection induces storms of abortion in pregnant cows and ewes, resulting in drastic economic losses because of fetal losses and culling of the dams. Herein, we evaluated the potential of recombinant protein of N. caninum dense granule protein 6 fused with glutathione-S-transferase (NcGRA6+GST) as a vaccine candidate against neosporosis in a pregnant mouse model. The protective efficacy was investigated by subcutaneous inoculation of BALB/c mice with recombinant NcGRA6+GST (25 pmol), and GST alone (25 pmol) or phosphate-buffered saline (PBS) as the controls. This study revealed the partial ability of NcGRA6+GST to protect the dams and offspring from N. caninum infection during the critical period of pregnancy. This ability was revealed by higher survival rate and lower parasite burden in brains of offspring of the NcGRA6+GST-immunized group in comparison with the control groups. In addition, mouse dams from NcGRA6+GST-immunized groups exhibited lower clinical score and minimum alteration in body weight in comparison with PBS or GST groups after challenge with N. caninum tachyzoites. Taken together, our results suggest the efficacy of recombinant NcGRA6 for interrupting the vertical transmission of N. caninum in mice by reducing the severity of infections in dams and offspring.
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影响因子：
4
作者：
Ramamoorthy, Sheela;Sanakkayala, Neelima;Sriranganathan, Nammalwar
通讯作者：
Sriranganathan, Nammalwar
影响因子：
4
作者：
Klevar, Siv;Kulberg, Siri;Olsen, Ingrid
通讯作者：
Olsen, Ingrid
影响因子：
5.5
作者：
Marugan-Hernandez, V.;Ortega-Mora, L. M.;Alvarez-Garcia, G.
通讯作者：
Alvarez-Garcia, G.
影响因子：
6.2
作者：
Gao, QL;Chen, NX;Field, EH
通讯作者：
Field, EH
影响因子：
5.5
作者：
Nishimura, Maki;Kohara, Junko;Nishikawa, Yoshifumi
通讯作者：
Nishikawa, Yoshifumi