Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes
Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes
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精子表面芳基硫酸酯酶A可以分散卵丘卵母细胞复合物的卵丘基质
DOI：
10.1002/jcp.21113
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发表时间：
2007
影响因子：
5.6
通讯作者：
N. Tanphaichitr
中科院分区：
文献类型：
--
作者：
Alexander Wu;Araya Anupriwan;S. Iamsaard;K. Chakrabandhu;Daniela Costa Santos;T. Rupar;B. Tsang;E. Carmona;N. Tanphaichitr
膨化积云卵母细胞复合物（COCs）的积云细胞层与透明质酸、硫酸软骨素B蛋白聚糖和连接蛋白网络相互连接，并可通过精子表面透明质酸酶分散。在本报告中，我们发现存在于精子头表面的芳基硫酸酯酶A （AS‐A）也具有这种分散作用。纯化的AS‐A不含蛋白酶、透明质酸酶和软骨素酶活性，可以分散膨胀COCs的积云基质。然而，使用对硝基儿茶酚硫酸盐（人工底物）检测COC分散作用与AS - A脱硫活性无关。在芹菜素（一种透明质酸酶抑制剂）的存在下，用抗AS - A IgG预处理的精子孵育COCs，孵育1小时后，COCs没有出现基质分散，而在用免疫前IgG预处理的精子孵育的COCs中，已经有几个积云层分散。此外，与野生型精子相比，来自AS‐A缺失小鼠的精子显示出COC分散的显著延迟。在精子- COC共孵育1小时内，与AS - A零精子孵育的COC尺寸为原始尺寸的65%，而与野生型精子孵育的COC尺寸仅为原始尺寸的17%。当芹菜素存在于共孵育中时，观察到AS‐A（−/−）小鼠精子的COC分散进一步延迟。我们还首次发现AS‐A对硫酸软骨素B具有特异性亲和力，硫酸软骨素B是积云基质蛋白聚糖网络的一个组成部分；这可能提供了精子表面AS - a诱导积云基质失稳的机制。j .细胞。中国生物医学工程学报，2009,31(2):391 - 391。©2007 Wiley‐Liss, Inc。
Cumulus cell layers of expanded cumulus oocyte complexes (COCs) are interlinked with networks of hyaluronic acid, chondroitin sulfate B proteoglycans and link proteins, and they can be dispersed by sperm surface hyaluronidases. In this report, we showed that arylsulfatase A (AS‐A), existing on the sperm head surface, also had this dispersion action. Purified AS‐A free of protease, hyaluronidase and chondroitinase activities could disperse the cumulus matrix of expanded COCs. However, this COC dispersion action was not associated with AS‐A desulfation activity, assayed by using p‐nitrocatecholsulfate (artificial substrate). COCs incubated for 1 h with sperm pretreated with anti‐AS‐A IgG in the presence of apigenin (a hyaluronidase inhibitor) did not exhibit matrix dispersion, whereas several cumulus layers were already dispersed in COCs incubated with sperm pretreated with preimmune IgG. Furthermore, sperm from AS‐A null mice showed a significant delay in COC dispersion, compared with wild‐type sperm. Within 1 h of sperm‐COC co‐incubation, the size of COCs incubated with AS‐A null sperm was 65% of the original dimension, whereas that of COCs inseminated with wild‐type sperm was only 17%. A further delay in COC dispersion by AS‐A(−/−) mouse sperm was observed when apigenin was present in the co‐incubation. We also showed for the first time that AS‐A had a specific affinity for chondroitin sulfate B, a component of cumulus matrix proteoglycan networks; this might provide a mechanism of cumulus matrix destabilization induced by sperm surface AS‐A. J. Cell. Physiol. 213: 201–211, 2007. © 2007 Wiley‐Liss, Inc.
兔睾丸 β-半乳糖苷酶和芳基硫酸酯酶 A 的表征：顶体反应过程中精子的纯化和定位。
影响因子：
3.6
作者：
Nikolajczyk,BS;O'Rand,MG
通讯作者：
O'Rand,MG