Characterization of rabbit testis beta-galactosidase and arylsulfatase A: purification and localization in spermatozoa during the acrosome reaction.
Characterization of rabbit testis beta-galactosidase and arylsulfatase A: purification and localization in spermatozoa during the acrosome reaction.
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兔睾丸 β-半乳糖苷酶和芳基硫酸酯酶 A 的表征：顶体反应过程中精子的纯化和定位。
DOI：
10.1095/biolreprod46.3.366
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发表时间：
1992
影响因子：
3.6
通讯作者：
O'Rand,MG
中科院分区：
文献类型：
--
作者：
Nikolajczyk,BS;O'Rand,MG
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Examination of the role of carbohydrates in specific recognition between spermatozoa and zona pellucida has focussed on understanding the interaction of sperm hydrolases or lectin-like molecules with zona pellucida ligands. To elucidate the role of specific spermatozoan hydrolases in gamete interaction, rabbit testis β-galactosidase and arylsulfatase A were purified, characterized, and localized in spermatozoa. β-Galactosidase and arylsulfatase A co-purified after affinity, size, or reverse-phase chromatography. N-Terminal amino acid analysis and enzymatic characterization suggested that neither enzyme is a testis-specific isozyme. Size chromatography indicated that both enzymes aggregated into macromolecular complexes at pH 4.0, while both dissociated at pH 8.0. β-Galactosidase and arylsulfatase A co-localized on the sperm surface and in the acrosome and postacrosomal regions of spermatozoa. Throughout the zona-induced acrosome reaction, both enzymes remained associated with the detached acrosomal cap and postacrosomal region of acrosome-reacted spermatozoa. Because the acrosome is an acidic subcellular compartment, internal β-galactosidase and arylsulfatase A are probably aggregated in acrosome-intact spermatozoa and dissociate as they are exposed to pH increases during the acrosome reaction.
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顶体中的溶酶体酶及其在受精中的可能作用。
DOI：
--
发表时间：
1970
期刊：
Journal of Reproduction and Fertility
影响因子：
--
作者：
A. Allison;E. Hartree
通讯作者：
E. Hartree
人肝脏甲基伞形基β-半乳糖苷酶的分离和表征。
DOI：
10.1042/bj1570189
发表时间：
1976
期刊：
The Biochemical journal
影响因子：
--
作者：
P. Cheetham;N. Dance
通讯作者：
N. Dance
ARBACIA 精子与 S35 标记的肥料之间的相互作用
DOI：
10.2307/1539538
发表时间：
1961
期刊：
The Biological Bulletin
影响因子：
--
作者：
R. R. Hathaway;C. B. Metz
通讯作者：
C. B. Metz
来自大鼠附睾液的β-半乳糖苷酶与附着在附睾膜上的识别位点结合，该识别位点不同于磷酸甘露糖基受体。
DOI：
10.1016/0006-291x(87)90319-6
发表时间：
1987
影响因子：
3.1
作者：
M. Sosa;L. Mayorga;F. Bertini
通讯作者：
F. Bertini
公牛精浆和生殖器官中的β-半乳糖苷酶。
DOI：
10.1016/0020-711x(86)90395-2
发表时间：
1986
期刊：
The International journal of biochemistry
影响因子：
--
作者：
A. Jauhiainen;T. Vanha
通讯作者：
T. Vanha