Nanofibrous Patches for Spinal Cord Regeneration.
Nanofibrous Patches for Spinal Cord Regeneration.
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DOI：
10.1002/adfm.200901889
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发表时间：
2010-05-10
影响因子：
19
通讯作者：
Li, Song
中科院分区：
文献类型：
--
作者：
Zhu, Yiqian;Wang, Aijun;Shen, Wenqian;Patel, Shyam;Zhang, Rong;Young, William L.;Li, Song
脊髓再生的困难与轴突生长的抑制因素和损伤区域缺乏适当的轴突引导有关。在这里，我们开发了具有排列的纳米纤维的支架，用于神经指导和脊髓药物输送。以聚L-乳酸(PLLA)和聚丙交酯-乙交酯共聚物(PLGA)为共混聚合物，制备了具有双层结构的纳米纤维支架材料：内层为取向纳米纤维，外层为无规纳米纤维。罗利普兰是一种可以增强神经元中cAMP活性并抑制炎症反应的小分子，它被固定在纳米纤维上。为了验证纳米纤维支架的治疗效果，将负载罗利普兰的纳米纤维支架用于桥接8周龄裸鼠的半横断损伤。含罗利普兰的支架通过支架和病变促进轴突生长，通过支架促进血管生成，并减少病变中星形胶质细胞和硫酸软骨素蛋白多糖的数量。运动评分分析显示，植入罗利普兰的支架3周后后肢功能明显改善。本研究表明，纳米纤维支架为脊髓再生提供了一个有价值的药物输送平台。
The difficulty in spinal cord regeneration is related to the inhibitory factors for axon growth and the lack of appropriate axon guidance in the lesion region. Here we developed scaffolds with aligned nanofibers for nerve guidance and drug delivery in spinal cord. Blended polymers including Poly (l-lactic acid) (PLLA) and Poly (lactide-co-glycolide) (PLGA) were used to electrospin nanofibrous scaffolds with two-layer structure: aligned nanofibers in the inner layer and random nanofibers in the outer layer. Rolipram, a small molecule that can enhance cAMP activity in neurons and suppress inflammatory responses, was immobilized onto nanofibers. To test the therapeutic effects of nanofibrous scaffolds, the nanofibrous scaffolds loaded with rolipram were used to bridge the hemisection lesion in 8-week old athymic rats. The scaffolds with rolipram increased axon growth through the scaffolds and in the lesion, promoted angiogenesis through the scaffold, and decreased the population of astrocytes and chondroitin sulfate proteoglycans in the lesion. Locomotor scale rating analysis showed that the scaffolds with rolipram significantly improved hindlimb function after 3 weeks. This study demonstrated that nanofibrous scaffolds offered a valuable platform for drug delivery for spinal cord regeneration.
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影响因子：
3.1
作者：
Boland, ED;Matthews, JA;Bowlin, GL
通讯作者：
Bowlin, GL
DOI：
10.1073/pnas.0900222106
发表时间：
2009-06-09
影响因子：
11.1
作者：
Dray, Cyril;Rougon, Genevieve;Debarbieux, Franck
通讯作者：
Debarbieux, Franck
DOI：
10.1073/pnas.0704581104
发表时间：
2007-07-17
影响因子：
11.1
作者：
Hashi, Craig K.;Zhu, Yiqian;Li, Song
通讯作者：
Li, Song
影响因子：
16.2
作者：
Neumann, S;Bradke, F;Basbaum, AI
通讯作者：
Basbaum, AI
影响因子：
5.3
作者：
Niederöst, BP;Zimmermann, DR;Bandtlow, CE
通讯作者：
Bandtlow, CE