Characterization of a method to study urodynamics and bladder nociception in male and female mice.
Characterization of a method to study urodynamics and bladder nociception in male and female mice.
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DOI：
10.1111/luts.12365
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Mickle AD
中科院分区：
文献类型：
--
作者：
Srivastava P;Lai HH;Mickle AD
膀胱膨胀引起的腹部肌电图或内脏运动反应（VMR）已被证实是一种测量小鼠膀胱痛觉的方法，但它并非没有局限性。本研究的目的是解决这些局限性，并验证排尿诱发的VMR作为膀胱痛觉小鼠的测量方法。我们用雄性和雌性C57BL/6小鼠，在膀胱内灌注0.5%醋酸前后，评估了细胞术诱导排尿的VMR反应。然后，我们给药膀胱内利多卡因，以确认VMR反应是伤害性的。对VMR及相关膀胱压力进行分析。我们发现，在雄性和雌性小鼠膀胱内连续输注液体均可诱发VMR。这种反应在膀胱损伤后增强，局部麻醉可以减弱，这为该方法可用于评估膀胱伤害感受提供了强有力的证据。此外，在VMR记录中获得的膀胱压力痕迹的评估显示，雄性或雌性小鼠膀胱损伤后的间缩间隔没有改变。然而，我们确实观察到雌性小鼠膀胱损伤后阈值压力峰值下降，这可以通过利多卡因恢复。总之，该技术可以测量雌雄小鼠与排尿相关的VMR和膀胱痛觉。尽管使用麻醉仍然存在混淆，但进一步探索非麻醉的空诱发VMR是有必要的。
Abdominal electromyogram or visceromotor response (VMR) elicited by bladder distension is a validated as a measure of bladder nociception in mice, however it is not without its limitations. The aim of this study is to address some of these limitations and validate voiding evoked VMR as a measure of bladder nociception mice. Using both male and female C57BL/6 mice we assessed the VMR response to cytometry- induced voiding before and after instillation of 0.5% acetic acid into the bladder. We then delivered intravesical lidocaine to confirm the VMR response as nociceptive. VMR and correlative cystometric bladder pressures were analyzed. We found that the VMR can be evoked by continuous fluid infusion into the bladder of both male and female mice. This response is potentiated after bladder injury and can be attenuated by administration of a local anesthetic, providing strong evidence that this method can be used to evaluate bladder nociception. Further, evaluation of cystometric pressure traces obtained during VMR recording revealed that intercontraction intervals were not altered after bladder injury in either male or female mice. However, we did observe a decrease in peak threshold pressures after bladder injury in female mice, which could be rescued by lidocaine administration. In conclusion, this technique can measure the VMR and bladder nociception associated with voiding in both female and male mice. Although confounds still exist with the use of anesthesia, further exploration of non-anesthetized voiding-evoked VMR is warranted.
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