ConfocalVR: Immersive Visualization for Confocal Microscopy.

ConfocalVR: Immersive Visualization for Confocal Microscopy.
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DOI:
10.1016/j.jmb.2018.06.035
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发表时间:
2018-10-19
影响因子:
5.6
通讯作者:
Skillman T
Skillman T
中科院分区:
生物学2区
文献类型:
--
作者:
Stefani C;Lacy-Hulbert A;Skillman T

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ConfocalVR是一个虚拟现实(VR)应用程序,旨在提高研究人员研究细胞结构复杂性的能力。共焦显微镜在不同的焦平面上拍摄荧光标记的蛋白质或分子的照片,以在整个标本中创建一组2D图像。当前的软件应用程序重建3D图像,并将其作为2D投影渲染到计算机屏幕上,其中用户需要旋转图像以暴露完整的3D结构。这个过程是精神负担,打破了如果你停止旋转,并没有利用眼睛的整个视野。ConfocalVR利用消费级虚拟现实(VR)系统,使用户完全沉浸在3D细胞图像中。在该虚拟环境中,用户可以:1)在不离开虚拟空间的情况下调整图像观看参数,2)伸出手并抓取图像以快速旋转和缩放图像以聚焦于关键特征,以及3)在共享虚拟空间中与其他用户交互,从而实现实时协作探索和讨论。我们发现,沉浸式VR技术允许用户快速了解细胞结构和蛋白质或分子分布。我们注意到,如果没有亲身体验,就不可能理解沉浸式可视化的价值,因此我们鼓励读者访问VR系统,下载该软件,并自行评估。ConfocalVR软件可在http://www.confocalvr.com下载,对非营利组织免费。
ConfocalVR is a virtual reality (VR) application created to improve the ability of researchers to study the complexity of cell architecture. Confocal microscopes take pictures of fluorescently labeled proteins or molecules at different focal planes to create a stack of 2D images throughout the specimen. Current software applications reconstruct the 3D image and render it as a 2D projection onto a computer screen where users need to rotate the image to expose the full 3D structure. This process is mentally taxing, breaks down if you stop the rotation, and does not take advantage of the eye’s full field of view. ConfocalVR exploits consumer-grade virtual reality (VR) systems to fully immerse the user in the 3D cellular image. In this virtual environment the user can: 1) adjust image viewing parameters without leaving the virtual space, 2) reach out and grab the image to quickly rotate and scale the image to focus on key features, and 3) interact with other users in a shared virtual space enabling real-time collaborative exploration and discussion. We found that immersive VR technology allows the user to rapidly understand cellular architecture and protein or molecule distribution. We note that it is impossible to understand the value of immersive visualization without experiencing it first hand, so we encourage readers to get access to a VR system, download this software, and evaluate it for yourself. The ConfocalVR software is available for download at http://www.confocalvr.com, and is free for nonprofits.
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