Brillouin micro-spectroscopy through aberrations via sensorless adaptive optics.

Brillouin micro-spectroscopy through aberrations via sensorless adaptive optics.
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DOI:
10.1063/1.5027838
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发表时间:
2018-04-16
影响因子:
4
通讯作者:
Scarcelli G
Scarcelli G
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Edrei E;Scarcelli G

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布里渊光谱是一种非接触式粘弹性表征的光学技术,最近在生物样品的三维映射中得到了应用。布里渊光谱学性能由于光学像差而迅速退化,因此仅限于均匀透明样品。在这项工作中,我们开发了一个自适应光学(AO)配置设计的布里渊散射光谱工程入射波前和校正像差。我们的配置不需要直接的波前传感和注射的“导星”,因此,它可以实现,而不需要样品的预处理。我们使用我们的AO-Brillouin光谱仪在像差的幻影和生物样品,并获得了提高的精度和分辨率的布里渊光谱分析,我们证明了2.5倍的增强布里渊信号强度和1.4倍的轴向分辨率的改善,因为光学像差的校正。
Brillouin spectroscopy is a powerful optical technique for non-contact viscoelastic characterizations which has recently found applications in three-dimensional mapping of biological samples. Brillouin spectroscopy performances are rapidly degraded by optical aberrations and have therefore been limited to homogenous transparent samples. In this work, we developed an adaptive optics (AO) configuration designed for Brillouin scattering spectroscopy to engineer the incident wavefront and correct for aberrations. Our configuration does not require direct wavefront sensing and the injection of a “guide-star”; hence, it can be implemented without the need for sample pre-treatment. We used our AO-Brillouin spectrometer in aberrated phantoms and biological samples and obtained improved precision and resolution of Brillouin spectral analysis; we demonstrated 2.5-fold enhancement in Brillouin signal strength and 1.4-fold improvement in axial resolution because of the correction of optical aberrations.
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