Genetic diversity of the Plasmodium falciparum apical membrane antigen I gene in parasite population from the China-Myanmar border area.
Genetic diversity of the Plasmodium falciparum apical membrane antigen I gene in parasite population from the China-Myanmar border area.
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中缅边境地区恶性疟原虫顶膜抗原I基因遗传多样性研究
DOI：
10.1016/j.meegid.2016.01.021
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发表时间：
2016-04
影响因子：
3.2
通讯作者：
Cui, Liwang
中科院分区：
文献类型：
--
作者：
Zhu, Xiaotong;Zhao, Zhenjun;Feng, Yonghui;Li, Peipei;Liu, Fei;Liu, Jun;Yang, Zhaoqing;Yan, Guiyun;Fan, Qi;Cao, Yaming;Cui, Liwang
为了解东南亚恶性疟原虫顶膜抗原1(PfAMA1)基因的遗传多样性，我们测定了采自中国-缅甸边境地区的13 5株恶性疟原虫分离株的PfAMA1基因序列，并与来自全球7个恶性疟原虫种群的95 6株PfAMA1基因序列进行了比较。分析表明，PfAMA1在全球恶性疟原虫种群中具有高度的遗传多样性，共鉴定出229种单倍型。中国-缅甸边境PfAMA1基因的遗传多样性在该基因的不同结构域上分布不均匀。中国-缅甸边境PfAMA1的序列多样性低于泰国、非洲和大洋洲种群，但高于南美种群。这似乎与不同疟疾流行地区的流行水平有很好的相关性，在这些地区，高流行地区有利于寄生虫分离株的遗传杂交和产生更高的遗传多样性。中和性测试表明，在中国-缅甸边境寄生虫种群中，PfAMA1的整个胞外域和结构域I都显著偏离中性值。我们发现证据支持恶性疟原虫种群之间存在实质性的大陆遗传结构，其中在中国-缅甸边境和南美种群之间检测到的遗传分化最高。虽然没有特定地区独有的等位基因，但主要等位基因及其频率在地理上存在相当大的差异，进一步突出了在疫苗设计中包括更多PfAMA1等位基因的必要性。
To investigate the genetic diversity of the Plasmodium falciparum apical membrane antigen 1 (PfAMA1) gene in Southeast Asia, we determined PfAMA1 sequences from 135 field isolates collected from the China-Myanmar border area and compared them with 956 publically available PfAMA1 sequences from seven global P. falciparum populations. This analysis revealed high genetic diversity of PfAMA1 in global P. falciparum populations with a total of 229 haplotypes identified. The genetic diversity of PfAMA1 gene from the China-Myanmar border is not evenly distributed in the different domains of this gene. Sequence diversity in PfAMA1 from the China-Myanmar border is lower than that observed in Thai, African and Oceanian populations, but higher than that in the South American population. This appeared to correlate well with the levels of endemicity of different malaria-endemic regions, where hyperendemic regions favor genetic cross of the parasite isolates and generation of higher genetic diversity. Neutrality tests show significant departure from neutrality in the entire ectodomain and Domain I of PfAMA1 in the China-Myanmar border parasite population. We found evidence supporting a substantial continent-wise genetic structure among P. falciparum populations, with the highest genetic differentiation detected between the China-Myanmar border and the South American populations. Whereas no alleles were unique to a specific region, there were considerable geographical differences in major alleles and their frequencies, highlighting further necessity to include more PfAMA1 alleles in vaccine designs.
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影响因子：
5.4
作者：
HILL W G;ROBERTSON A
通讯作者：
ROBERTSON A
影响因子：
3
作者：
Garg S;Alam MT;Das MK;Dev V;Kumar A;Dash AP;Sharma YD
通讯作者：
Sharma YD
影响因子：
3.6
作者：
Healer, J;Murphy, V;Batchelor, A
通讯作者：
Batchelor, A
恶性疟原虫裂殖子中棒状体颈蛋白 RON2 与微线体蛋白 AMA1 形成复合物
影响因子：
1.9
作者：
Cao, Jun;Kaneko, Osamu;Torii, Motomi
通讯作者：
Torii, Motomi
影响因子：
1.5
作者：
Eisen, DP;Marshall, VM;Coppel, RL
通讯作者：
Coppel, RL