Divergent transcription from active promoters.
Divergent transcription from active promoters.
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DOI：
10.1126/science.1162253
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发表时间：
2008-12-19
期刊：
影响因子：
--
通讯作者：
Sharp PA
中科院分区：
文献类型：
--
作者：
Seila AC;Calabrese JM;Levine SS;Yeo GW;Rahl PB;Flynn RA;Young RA;Sharp PA
RNA聚合酶II（RNAPII）的起始被认为是从大多数基因单向发生的。我们已经确定了哺乳动物细胞中的一类短RNA，挑战这一惯例，这表明存在广泛的分歧转录。转录起始位点相关RNA（TSSa-RNA）是由活性蛋白质编码基因启动子分化转录的，其反义和正义TSSa-RNA峰分别位于TSS的上游和下游。转录起始标记如结合的RNAPII和H3 K4-三甲基化组蛋白位于正义和反义TSSa-RNA位置;然而，H3 K79-二甲基化组蛋白，延伸RNAPII的标志，存在于TSS的单向下游。这些结果表明，短距离的转录差异是活性启动子的常见现象，并且可能有助于启动子区域保持为后续调节做好准备的状态。
Initiation by RNA polymerase II (RNAPII) is thought to occur unidirectionally from most genes. We have identified a class of short RNAs in mammalian cells that challenges this convention, suggesting the existence of widespread divergent transcription. Transcription start site-associated RNAs (TSSa-RNAs) are transcribed divergently from active protein-coding gene promoters, with anti-sense and sense TSSa-RNA peaks located upstream and downstream of the TSS, respectively. Transcription initiation marks such as bound RNAPII and H3K4-trimethylated histones are located at both sense and anti-sense TSSa-RNA positions; however, H3K79-dimethylated histones, a signature of elongating RNAPII, are present unidirectionally downstream of TSSs. These results suggest that divergent transcription over short distances is common of active promoters, and may help promoter regions maintain a state poised for subsequent regulation.
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