A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II.
A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II.
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DOI：
10.1242/jcs.141788
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发表时间：
2014-03-01
影响因子：
4
通讯作者：
Lamond AI
中科院分区：
文献类型：
--
作者：
Hutten S;Chachami G;Winter U;Melchior F;Lamond AI
Cajal小体是参与snRNPs和snoRNPs生物发生、端粒维持和组蛋白mRNA加工的核结构。最近，SUMO异肽酶USPL1被鉴定为Cajal小体的一个组成部分，对细胞生长和Cajal小体的完整性至关重要。然而，到目前为止，USPL1的细胞功能尚不清楚。在这里，我们在人类细胞中使用rnai介导的敲低，结合生化和荧光显微镜方法来研究USPL1的功能及其与Cajal小体的联系。我们证明，当USPL1基因被敲低时，RNA聚合酶（RNAP） II转录的snrna水平会降低，snRNP组装和pre-mRNA剪接等下游过程也会受到损害。重要的是，我们发现USPL1直接与U snRNA位点相关联，并与参与rnapii介导的snRNA转录的小延伸复合物组分相互作用和共定位。因此，我们的数据表明，USPL1在rnapii介导的snRNA转录中起着关键作用。
Cajal bodies are nuclear structures that are involved in biogenesis of snRNPs and snoRNPs, maintenance of telomeres and processing of histone mRNA. Recently, the SUMO isopeptidase USPL1 was identified as a component of Cajal bodies that is essential for cellular growth and Cajal body integrity. However, a cellular function for USPL1 is so far unknown. Here, we use RNAi-mediated knockdown in human cells in combination with biochemical and fluorescence microscopy approaches to investigate the function of USPL1 and its link to Cajal bodies. We demonstrate that levels of snRNAs transcribed by RNA polymerase (RNAP) II are reduced upon knockdown of USPL1 and that downstream processes such as snRNP assembly and pre-mRNA splicing are compromised. Importantly, we find that USPL1 associates directly with U snRNA loci and that it interacts and colocalises with components of the Little Elongation Complex, which is involved in RNAPII-mediated snRNA transcription. Thus, our data indicate that USPL1 plays a key role in RNAPII-mediated snRNA transcription.
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影响因子：
9.2
作者：
Andersen, JS;Lyon, CE;Lamond, AI
通讯作者：
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