Complementary serum proteomic analysis of autoimmune hepatitis in mice and patients.
Complementary serum proteomic analysis of autoimmune hepatitis in mice and patients.
复制标题
小鼠和患者自身免疫性肝炎的补充血清蛋白质组学分析
DOI：
10.1186/1479-5876-11-146
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发表时间：
2013-06-13
影响因子：
7.4
通讯作者：
Liang S
中科院分区：
文献类型：
--
作者：
Li H;Li G;Zhao X;Wu Y;Ma W;Liu Y;Gong F;Liang S
BackgroundAutoimmune hepatitis (AIH) is a chronic liver disease caused by inflammation of the liver. The etiology of AIH remains elusive, and there are no reliable serum biomarkers.MethodsIn order to identify candidate biomarkers, 2-DE analysis of serum proteins was performed using a mouse model of AIH induced by treatment with concanavalin A (ConA). To enrich samples for low abundance molecules a commercial albumin removal reagent was used. In an independent analysis, candidate biomarkers were identified in AIH patient’s serum by a targeted iTRAQ (isobaric tags for relative and absolute quantification) identification. Candidates were validated in independent cohorts of ConA treated mice and AIH patients by ELISA (enzyme-linked immuno sorbent assay).ResultsNine proteins were differentially expressed in AIH mice treated with con-A. Two of these, the third component of complement (C3) and alpha-2-macroglobulin (A2M) were also up-regulated in AIH patient’s sera by a targeted iTRAQ identification. In separate validation studies, serum C3 and A2M levels were increased in mice with ConA treatment after 20-40 h and in 34 AIH patients in a subgroup analysis, females with AIH aged 20–50 years old displayed the largest increases in serum A2M level. Biological network analysis implements the complement cascade and protease inhibitors in the pathogenesis of AIH.ConclusionThe serum proteins C3 and A2M are increased both in a mouse model and in patients with AIH by both 2-DE and iTRAQ methods. This integrated serum proteomics investigation should be applicable for translational researchers to study other medical conditions.
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影响因子：
5.6
作者：
Aparicio JL;Peña C;Retegui LA
通讯作者：
Retegui LA
影响因子：
4.4
作者：
Chong, Poh Kuan;Lee, Huiyin;Zhou, Jianbiao;Liu, Shaw-Cheng;Loh, Marie Chiew Shia;Wang, Ting Ting;Chan, Siew Pang;Smoot, Duane T.;Ashktorab, Hassan;So, Jimmy Bok Yan;Lim, Khong Hee;Yeoh, Khay Guan;Lim, Yoon Pin
通讯作者：
Lim, Yoon Pin
影响因子：
3.4
作者：
Ogata, Yuko;Charlesworth, M. Cristine;Muddiman, David C.
通讯作者：
Muddiman, David C.
影响因子：
2.9
作者：
Liang, Shufang;Xu, Yuhuan;Wei, Yuquan
通讯作者：
Wei, Yuquan
DOI：
10.1073/pnas.0903602106
发表时间：
2009-09-01
影响因子：
11.1
作者：
Stegbauer, Johannes;Lee, De-Hyung;Linker, Ralf A.
通讯作者：
Linker, Ralf A.