Unraveling the structure and biological functions of RNA triple helices.
Unraveling the structure and biological functions of RNA triple helices.
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DOI：
10.1002/wrna.1598
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Brown JA
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文献类型：
--
作者：
Brown JA
自 Gary Felsenfeld、David Davies 和 Alexander Rich 首次在体外表征 RNA 三螺旋以来，已有近 63 年了。 RNA 三螺旋由三条链组成：沃森-克里克 RNA 双螺旋，其主凹槽与所谓的“第三链”建立氢键。在过去的 15 年里，人们已经认识到这些大沟 RNA 三螺旋，如单链和双链 RNA，也在细胞内发挥着重要的生物学作用。迄今为止，已知这些三螺旋在端粒合成和 RNA 剪接过程中介导催化作用，与配体和离子结合，以便代谢物传感核糖开关可以调节基因表达，并提供一种巧妙的策略来保护 RNA 3' 端免遭降解。由于 RNA 三螺旋在生物学中发挥着重要作用，人们对更好地了解 RNA 三螺旋的基本特性和开发其高通量发现方法产生了新的兴趣。这篇综述概述了主沟RNA三螺旋的基本生化和结构特性，总结了天然存在的RNA三螺旋的结构和功能，并描述了分离RNA三螺旋作为建立“三螺旋组”的手段的前瞻性策略。 RNA 结构和动力学 > RNA 结构和动力学 RNA 结构和动力学 > RNA 结构、动力学和化学 RNA 结构和动力学 > RNA 结构在生物系统中的影响 本文分类如下：天然存在的 RNA 三螺旋的三维结构显示为蓝色、紫色和绿色表示的三条链。总的来说，这些 RNA 三螺旋在催化、与配体结合（橙色）和稳定 RNA 方面发挥着重要的生物学作用。
It has been nearly 63 years since the first characterization of an RNA triple helix in vitro by Gary Felsenfeld, David Davies, and Alexander Rich. An RNA triple helix consists of three strands: A Watson–Crick RNA double helix whose major‐groove establishes hydrogen bonds with the so‐called “third strand”. In the past 15 years, it has been recognized that these major‐groove RNA triple helices, like single‐stranded and double‐stranded RNA, also mediate prominent biological roles inside cells. Thus far, these triple helices are known to mediate catalysis during telomere synthesis and RNA splicing, bind to ligands and ions so that metabolite‐sensing riboswitches can regulate gene expression, and provide a clever strategy to protect the 3′ end of RNA from degradation. Because RNA triple helices play important roles in biology, there is a renewed interest in better understanding the fundamental properties of RNA triple helices and developing methods for their high‐throughput discovery. This review provides an overview of the fundamental biochemical and structural properties of major‐groove RNA triple helices, summarizes the structure and function of naturally occurring RNA triple helices, and describes prospective strategies to isolate RNA triple helices as a means to establish the “triplexome”. RNA Structure and Dynamics > RNA Structure and Dynamics RNA Structure and Dynamics > RNA Structure, Dynamics and Chemistry RNA Structure and Dynamics > Influence of RNA Structure in Biological Systems This article is categorized under: The three‐dimensional structures of naturally occurring RNA triple helices are displayed with the three strands denoted in blue, purple, and green. Collectively, these RNA triple helices play important biological roles in catalysis, binding to ligands (orange), and stabilizing RNA.
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影响因子：
7
作者：
Buske FA;Bauer DC;Mattick JS;Bailey TL
通讯作者：
Bailey TL
影响因子：
64.8
作者：
Bertram, Karl;Agafonov, Dmitry E.;Luehrmann, Reinhard
通讯作者：
Luehrmann, Reinhard
DOI：
10.1038/newbio244099a0
发表时间：
1973-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
ARNOTT, S;BOND, PJ
通讯作者：
BOND, PJ
影响因子：
14.9
作者：
Bao P;Boon KL;Will CL;Hartmuth K;Lührmann R
通讯作者：
Lührmann R
影响因子：
16.8
作者：
Brown JA;Bulkley D;Wang J;Valenstein ML;Yario TA;Steitz TA;Steitz JA
通讯作者：
Steitz JA