Resurrection of Dormant Daphnia magna: Protocol and Applications.
Resurrection of Dormant Daphnia magna: Protocol and Applications.
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DOI：
10.3791/56637
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发表时间：
2018-01-19
期刊：
影响因子：
--
通讯作者：
Orsini L
中科院分区：
文献类型：
--
作者：
Cuenca Cambronero M;Orsini L
长期研究可以确定在较长时期内发生的生态进化过程。此外，它们还提供了关键的经验数据，可用于预测模型，以预测自然生态系统对未来环境变化的进化反应。然而，除了少数例外情况外，由于与获取时间样本相关的后勤困难，长期研究很少。时间动力学经常在实验室或控制的中生态实验中进行研究，这些实验具有重建野生自然种群进化的特殊研究。本文提供了一个标准操作程序（SOP）来恢复或复活休眠的大水蚤（Daphnia magna），这是水生生态系统中广泛存在的浮游动物关键物种，以极大地推进自然系统中最先进的纵向数据收集。复活生态学领域是在1999年由Kerfoot和他的同事定义的，尽管第一次孵化滞育浮游动物卵的尝试可以追溯到20世纪80年代末。自从Kerfoot的开创性论文发表以来，复活浮游动物物种的方法得到了越来越多的应用，尽管只是通过直接的知识转移在实验室中传播。这里，描述了一个SOP，提供了一个循序渐进的协议，在实践中复活休眠的大水蚤卵。本文提供了两个关键的研究，其中测量了复活的大水蚤种群对变暖的适应性反应，利用了在相同环境下研究历史和现代种群的能力。最后，讨论了下一代测序技术在恢复或休眠阶段的应用。这些技术为剖析进化过程和机制提供了前所未有的力量，如果将其应用于经历了长期选择压力变化的种群。
Long-term studies enable the identification of eco-evolutionary processes that occur over extended time periods. In addition, they provide key empirical data that may be used in predictive modelling to forecast evolutionary responses of natural ecosystems to future environmental changes. However, excluding a few exceptional cases, long-term studies are scarce because of logistic difficulties associated with accessing temporal samples. Temporal dynamics are frequently studied in the laboratory or in controlled mesocosm experiments with exceptional studies that reconstruct the evolution of natural populations in the wild. Here, a standard operating procedure (SOP) is provided to revive or resurrect dormant Daphnia magna, a widespread zooplankton keystone species in aquatic ecosystems, to dramatically advance the state-of-the-art longitudinal data collection in natural systems. The field of Resurrection Ecology was defined in 1999 by Kerfoot and co-workers, even though the first attempts at hatching diapausing zooplankton eggs date back to the late 1980s. Since Kerfoot's seminal paper, the methodology of resurrecting zooplankton species has been increasingly frequently applied, though propagated among laboratories only via direct knowledge transfer. Here, an SOP is described that provides a step-by-step protocol on the practice of resurrecting dormant Daphnia magna eggs. Two key studies are provided in which the fitness response of resurrected Daphnia magna populations to warming is measured, capitalizing on the ability to study historical and modern populations in the same settings. Finally, the application of next generation sequencing technologies to revived or still dormant stages is discussed. These technologies provide unprecedented power in dissecting the processes and mechanisms of evolution if applied to populations that have experienced changes in selection pressure over time.
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DOI：
10.1073/pnas.94.17.9171
发表时间：
1997-08-19
影响因子：
11.1
作者：
Caceres, CE
通讯作者：
Caceres, CE
影响因子：
2.6
作者：
APPLEBY, PG;NOLAN, PJ;BATTARBEE, RW
通讯作者：
BATTARBEE, RW
DOI：
10.1073/pnas.0702196104
发表时间：
2007-08-14
影响因子：
11.1
作者：
Bidle, Kay D.;Lee, SangHoon;Falkowski, Paul G.
通讯作者：
Falkowski, Paul G.
影响因子：
7.7
作者：
Agostini, Cecilia;Agudelo, P. A.;Zulaiha, A. R.
通讯作者：
Zulaiha, A. R.
影响因子：
2.6
作者：
Kilham, SS;Kreeger, DA;Herrera, L
通讯作者：
Herrera, L