Pregnancy-Induced Physiologic Adaptation of the Abdominal Aorta Is Associated with Changes in Gene Expression and Genomic Methylation.
Pregnancy-Induced Physiologic Adaptation of the Abdominal Aorta Is Associated with Changes in Gene Expression and Genomic Methylation.
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DOI：
10.1159/000493682
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发表时间：
2018
影响因子：
1.7
通讯作者：
Brown S
中科院分区：
文献类型：
--
作者：
Gelinne A;Brown L;Ko NL;Osol G;Brown S
10 - 11-易位（泰特），一种DNA去甲基化酶，已被确定为血管平滑肌细胞可塑性的主要表观遗传调节因子，可改变妊娠主动脉的生物力学。我们假设妊娠将诱导与泰特基因表达相关的主动脉口径和扩张性的显著适应性变化。解剖妊娠和非妊娠小鼠的腹部动脉并插管。使用压力伺服系统调节管腔内压力，同时使用视频尺寸分析仪测量管腔直径。qPCR和免疫印迹用于分析泰特基因的表达。用LUMA测定评估整体基因组甲基化。与NP对照组（706 ± 8 μm）相比，E18.5（836 ± 14 μm）和PP 30（889 ± 16 μm）小鼠的主动脉管腔直径显著增加。E18.5小鼠的扩张性降低（90 ± 4%），PP 30小鼠的扩张性恢复至NP（108 ± 2%）值（108 ± 3%）。Tet 2转录在妊娠初期下降，随后在妊娠晚期增加;与整体甲基化的变化呈负相关。主动脉的生理变化伴随着基因表达和基因组甲基化的变化，表明妊娠期间母体血管重塑的表观遗传成分。
Ten-Eleven-Translocation (Tet), a DNA demethylase enzyme, has been identified as a master epigenetic regulator of vascular smooth muscle cell plasticity that could alter the biomechanics of the pregnant aorta. We hypothesized that pregnancy will induce significant adaptive changes in aortic caliber and distensibility that correlate to Tet gene expression. Abdominal aortas from pregnant and non-pregnant mice were dissected and cannulated. Intraluminal pressure was adjusted using a pressure-servo system while using a video dimension analyzer to measure lumen diameter. qPCR and immunoblot was used to analyze expression of Tet genes. Global genomic methylation was assessed with the LUMA assay. Compared to the NP (706 ± 8 μm) control group, aortic luminal diameter was significantly increased in both E18.5 (836 ± 14 μm) and PP30 (889 ± 16 μm) mice. Distensibility was reduced in E18.5 (90 ± 4%) mice and returned to NP (108 ± 2%) values in PP30 (108 ± 3%) mice. Tet2 transcription decreased in the beginning of pregnancy and subsequently increased in late gestation; inversely corresponding to changes in global methylation. Physiologic changes in the aorta are accompanied by changes in gene expression and genomic methylation, suggesting an epigenetic component to maternal vascular remodeling during pregnancy.
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影响因子：
37.8
作者：
Liu R;Jin Y;Tang WH;Qin L;Zhang X;Tellides G;Hwa J;Yu J;Martin KA
通讯作者：
Martin KA
影响因子：
8.3
作者：
Evans, Caroline S.;Gooch, Linda;Shroff, Sanjeev G.
通讯作者：
Shroff, Sanjeev G.
影响因子：
2.4
作者：
Clark, T. E.;Lillie, M. A.;Shadwick, R. E.
通讯作者：
Shadwick, R. E.
DOI：
10.1016/0002-9378(86)90477-1
发表时间：
1986-04-01
影响因子：
9.8
作者：
HART, MV;MORTON, MJ;METCALFE, J
通讯作者：
METCALFE, J
DOI：
10.1152/ajpheart.1998.274.5.h1605
发表时间：
1998-05-01
影响因子：
4.8
作者：
Edouard, DA;Pannier, BM;Safar, ME
通讯作者：
Safar, ME