The role of carbonic anhydrase-mediated tissue oxygen extraction in a marine teleost acclimated to hypoxia.
The role of carbonic anhydrase-mediated tissue oxygen extraction in a marine teleost acclimated to hypoxia.
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碳酸酐酶介导的组织氧提取在适应缺氧的海洋硬骨鱼中的作用。
DOI：
10.1242/jeb.244474
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
A. Esbaugh
中科院分区：
文献类型：
--
作者：
Angelina M. Dichiera;Benjamin Negrete;K. L. Ackerly;A. Esbaugh
随着低氧(O2 Level≤2 mg L-1)在水生和海洋生态系统中的日益普遍，人们对鱼类在应激环境中改善其表现的适应机制越来越感兴趣。在这里，我们研究了一种拟议的提高组织氧提取的策略-血浆可访问的碳酸氢酶(CA-IV)-在低氧条件下对一种在波动的栖息地中茁壮成长的河口鱼类(红鱼，Sciaenopsocellatus)的贡献。我们预测，低氧习服的鱼将增加CA-IV在要求有氧的组织中的流行率，以提供更有效的组织氧提取。此外，我们预测，与未适应低氧的鱼类相比，低氧习服对组织氧提取的表型变化可能会改善100%氧气条件下(即常氧)下的呼吸和游泳表现。有趣的是，两种处理之间CA-IV的相对mRNA表达、蛋白丰度和酶活性没有显著差异，表明CA-IV功能在低氧条件下保持不变。同样，在常氧条件下进行测试时，低氧驯化的鱼的呼吸性能与对照鱼相似。低氧驯化的鱼类的临界游泳速度(Ucrit)显著较高，但转化为边际生态效益，每秒增加∼0.3体长。相反，低氧习服的鱼在游泳试验中可能更多地依赖无氧代谢，利用突发性游泳的时间是对照鱼的1.5倍。虽然CA-IV的维持可能仍然是耐缺氧的一个重要因素，但我们的证据表明，缺氧习服的红鼓正在使用其他机制来应对氧气耗尽的环境。
With the growing prevalence of hypoxia (O2 levels ≤2 mg l-1) in aquatic and marine ecosystems, there is increasing interest in the adaptive mechanisms fish may employ to better their performance in stressful environments. Here, we investigated the contribution of a proposed strategy for enhancing tissue O2 extraction - plasma-accessible carbonic anhydrase (CA-IV) - under hypoxia in a species of estuarine fish (red drum, Sciaenops ocellatus) that thrives in fluctuating habitats. We predicted that hypoxia-acclimated fish would increase the prevalence of CA-IV in aerobically demanding tissues to confer more efficient tissue O2 extraction. Furthermore, we predicted the phenotypic changes to tissue O2 extraction that occur with hypoxia acclimation may improve respiratory and swim performance under 100% O2 conditions (i.e. normoxia) when compared with performance in fish that have not been acclimated to hypoxia. Interestingly, there were no significant differences in relative CA-IV mRNA expression, protein abundance or enzyme activity between the two treatments, suggesting CA-IV function is maintained under hypoxia. Likewise, respiratory performance of hypoxia-acclimated fish was similar to that of control fish when tested in normoxia. Critical swim speed (Ucrit) was significantly higher in hypoxia-acclimated fish but translated to marginal ecological benefits with an increase of ∼0.3 body lengths per second. Instead, hypoxia-acclimated fish may have relied more heavily on anaerobic metabolism during their swim trials, utilizing burst swimming 1.5 times longer than control fish. While the maintenance of CA-IV may still be an important contributor for hypoxia tolerance, our evidence suggests hypoxia-acclimated red drum are using other mechanisms to cope in an O2-depleted environment.
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Whitney,PL;Briggle,TV
通讯作者：
Briggle,TV
影响因子：
7.7
作者：
Damsgaard, Christian;Lauridsen, Henrik;Brauner, Colin J.
通讯作者：
Brauner, Colin J.
影响因子：
2.8
作者：
Esbaugh, Andrew J.;Ackerly, Kerri L.;Negrete, Benjamin
通讯作者：
Negrete, Benjamin
红细胞碳酸酐酶通过红石首鱼的根效应介导氧气输送
影响因子：
2.8
作者：
Dichiera, Angelina M.;Esbaugh, Andrew J.
通讯作者：
Esbaugh, Andrew J.