Pulp-capping with Recombinant Human Insulin- like Growth Factor I (rhIGF-I) in Rat Molars
Pulp-capping with Recombinant Human Insulin- like Growth Factor I (rhIGF-I) in Rat Molars
复制标题
用重组人胰岛素样生长因子 I (rhIGF-I) 对大鼠磨牙进行盖髓
DOI：
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发表时间：
2001
影响因子：
--
通讯作者：
A. Flyvbjerg
中科院分区：
文献类型：
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作者：
H. Løvschall;O. Fejerskov;A. Flyvbjerg
本研究的目的是探索使用重组人胰岛素样生长因子 I (rhIGF-I) 盖髓后的牙髓愈合和修复性牙本质生成。通过两个月大 Wistar 大鼠第一上磨牙的近中牙髓角进行曝光。在对侧对照中，用含有 400 ng rhIGF-I 或盐水的一剂无菌 4% 甲基纤维素凝胶覆盖牙髓。用无菌聚四氟乙烯膜封闭暴露部位，并用IRM水泥填充空腔。额外的臼齿用 Dycal 盖住作为对照。 3、7或28天后，将动物麻醉并通过血管内灌注戊二醛固定。对磨牙进行脱钙和处理以进行组织学分析，并用 IRM 中的膜和残留甲基丙烯酸酯进行原位切割。仅包括根据盲法显微镜控制具有可接受的牙髓密封的样本。第3天，在使用rhIGF-I凝胶或对照凝胶的磨牙中观察到上牙髓中相同的炎症反应。第 7 天，两组的肉芽组织向内生长均部分取代了炎症浸润。 28天后，更频繁地观察到完整的牙本质桥接和管状牙本质形成，并且更接近含有rhIGF-I的测试物质。 rhIGF-I 封盖后的修复性牙本质反应与使用 Dycal 后的反应相似。总之，原位膜密封的微观控制提供了有关生长因子更微妙的牙髓影响的有效信息。观察结果表明，与媒介物对照相比，通过rhIGF-I对大鼠磨牙进行盖髓可增强修复性牙本质发生。
The aim of this study was to explore pulp healing and reparative dentinogenesis following pulp-capping by using recombinant human insulin-like growth factor I (rhIGF-I). Exposures were made through the mesial pulp horn in first upper molars in two-month-old Wistar rats. The pulp was covered with one dose of sterile 4% methylcellulose gel containing either 400 ng rhIGF-I or saline in contralateral controls. The exposure site was closed with sterile Teflon membrane, and the cavity was filled with IRM cement. Additional molars were capped with Dycal as controls. After 3, 7, or 28 days, animals were anesthetized and fixed by intravascular glutaraldehyde perfusion. Molars were decalcified and processsed for histological analysis and cut with membrane and residual methacrylate from IRM in situ. Only specimens with acceptable pulp sealing according to blinded microscopy control were included. On day 3, identical inflammatory responses in the upper pulp were observed in molars with rhIGF-I gel or control gel. On day 7, granulation tissue ingrowth had partly replaced inflammatory infiltration in both groups. After 28 days, complete dentin bridging and tubular dentin formation were observed more frequently and closer to the test substance containing rhIGF-I. The reparative dentin response to capping with rhIGF-I was similar to that after the use of Dycal. In conclusion, microscopic control of membrane sealing in situ gives valid information on the more subtle pulp effects of growth factors. The observations suggest that pulp-capping of rat molars by means of rhIGF-I enhances reparative dentinogenesis in comparison with vehicle controls.
影响因子：
4.2
作者：
Hu, CC;Zhang, CH;Tatum, NB
通讯作者：
Tatum, NB