Use of the interior cavity of the P22 capsid for site-specific initiation of atom-transfer radical polymerization with high-density cargo loading.
Use of the interior cavity of the P22 capsid for site-specific initiation of atom-transfer radical polymerization with high-density cargo loading.
复制标题
作者：
病毒样颗粒（VLP）已成为重要的和多功能的化学操纵的功能性杂化纳米结构的发展架构。在这里，我们已经成功地证明了原子转移自由基聚合（ATRP）反应的位点选择性引发，以形成一个可寻址的聚合物限制在一个VLP的内腔。P22和交联聚（甲基丙烯酸2-氨基乙酯）的这种蛋白质-聚合物杂化物可能用作用于包封和递送小分子货物的新的高密度递送载体。特别地，包封的聚合物可以充当用于连接感兴趣的伯胺反应性分子（例如荧光素染料或Gd-DTPA MRI造影剂）的支架。使用这种方法，与VLP的先前修饰相比，可以实现VLP的标记密度的显著增加。这些结果突出了多聚体蛋白质-聚合物缀合物在开发基于VLP的MRI造影剂中的潜在效用，其具有装载其他货物的可能性。
Virus-like particles (VLPs) have emerged as important and versatile architectures for chemical manipulation in the development of functional hybrid nanostructures. Here we have successfully demonstrated the site selective initiation of atom transfer radical polymerization (ATRP) reactions to form an addressable polymer constrained within the interior cavity of a VLP. This protein-polymer hybrid, of P22 and crosslinked poly(2-aminoethyl methacrylate), is potentially useful as a new high-density delivery vehicle for encapsulation and delivery of small molecule cargos. In particular, the encapsulated polymer can act as a scaffold for the attachment of primary amine reactive molecules of interest, such as a fluorescein dye or a Gd-DTPA MRI contrast agent. Using this approach, a significant increase in labeling density of the VLP, compared to previous modifications of VLPs, can be achieved. These results highlight the use of multimeric protein-polymer conjugates for their potential utility in the development of VLP-based MRI contrast agents with the possibility of loading other cargos.
登录
查看更多内容
影响因子：
10.8
作者：
Liepold LO;Abedin MJ;Buckhouse ED;Frank JA;Young MJ;Douglas T
通讯作者：
Douglas T
影响因子：
15
作者：
Abe, Satoshi;Hirata, Kunio;Watanabe, Yoshihito
通讯作者：
Watanabe, Yoshihito
影响因子：
10.8
作者：
Hooker, Jacob M.;Datta, Ankona;Francis, Matthew B.
通讯作者：
Francis, Matthew B.
影响因子：
4.9
作者：
Lucon, Janice;Abedin, Md Joynal;Douglas, Trevor
通讯作者：
Douglas, Trevor
影响因子：
--
作者：
Aniagyei, Stella E.;DuFort, Christopher;Dragnea, Bogdan
通讯作者：
Dragnea, Bogdan