Cloning and expression in Escherichia coli K-12 of the genes for major outer membrane protein OmpA from Shigella dysenteriae, Enterobacter aerogenes, and Serratia marcescens
Cloning and expression in Escherichia coli K-12 of the genes for major outer membrane protein OmpA from Shigella dysenteriae, Enterobacter aerogenes, and Serratia marcescens
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来自痢疾志贺氏菌、产气肠杆菌和粘质沙雷氏菌的主要外膜蛋白 OmpA 基因在大肠杆菌 K-12 中的克隆和表达
DOI：
10.1128/jb.149.1.145-150.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
U. Henning
中科院分区：
文献类型：
--
作者：
S. Cole;I. Sonntag;U. Henning
许多革兰氏阴性细菌的外膜含有一种主要的热修饰蛋白，该蛋白与大肠杆菌K-12的OmpA蛋白具有血清学交叉反应。利用克隆的大肠杆菌K12蛋白基因作为DNA-DNA杂交探针，我们能够从志贺菌中鉴定出相应的基因。产气肠杆菌和粘质沙雷氏菌。将其克隆到噬菌体Lambda载体中，并在大肠杆菌K-12中进行了表达研究。这三种OmpA蛋白都得到了充分表达，并正确地输出到外膜。在几个案例中，观察到大肠杆菌K-12蛋白的已知功能完全或部分恢复。
The outer membranes of many gram-negative bacteria contain a major heat-modifiable protein which shows serological cross-reactivity with the OmpA protein of Escherichia coli K-12. Using the cloned gene for the E. coli K12 protein as a DNA-DNA hybridization probe, we were able to identify the corresponding genes from Shigella dysenteriae. Enterobacter aerogenes, and Serratia marcescens. These were cloned in a phage lambda vector, and their expression in E. coli K-12 was studied. All three OmpA proteins were fully produced and correctly exported to the outer membrane. In several cases, complete or partial restoration of known function of the E. coli K-12 protein was observed.
OmpA 蛋白的基因结构，OmpA 蛋白是细胞与细胞相互作用所需的大肠杆菌的主要表面蛋白。
影响因子：
5.6
作者：
Movva,NR;Nakamura,K;Inouye,M
通讯作者：
Inouye,M