Small volatile lipophilic molecules induced belowground by aphid attack elicit a defensive response in neighbouring un-infested plants.
Small volatile lipophilic molecules induced belowground by aphid attack elicit a defensive response in neighbouring un-infested plants.
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DOI：
10.3389/fpls.2023.1154587
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
在植物与蚜虫相互作用的开创性研究中，我们观察到被蚜虫侵染的蚕豆植物可以通过根际传递信号，从而诱导完整的邻近植物的地上防御。蚜虫寄生蜂 Aphidius ervi 明显被水培溶液中生长的完整蚕豆植物所吸引，该溶液先前含有被豌豆蚜侵染的植物。为了识别可能介导这种地下植物与植物通讯的根际信号，使用固相萃取 (SPE) 从水培生长的 10 天龄的被豌豆侵染和未侵染的蚕豆植物中收集根分泌物。为了验证这些根分泌物触发针对蚜虫的防御机制的能力，我们将它们添加到水培溶液中生长的蚕豆植物中，并在风洞生物测定中测试这些植物，以评估它们对蚜虫寄生蜂 A. ervi 的吸引力。我们在豌豆蚜侵染的蚕豆植物的 SPE 提取物中鉴定出三种挥发性亲脂性小分子作为植物防御诱导子：1-octen-3-ol、sulcatone 和 sulcatol。在风洞试验中，我们记录到，与在用乙醇处理的水培溶液中生长的植物（对照）相比，在用这些化合物处理的水培溶液中生长的蚕豆植物对 A. ervi 的吸引力显着增加。 1-辛烯-3-醇和硫醇醇分别在3位和2位具有不对称取代的碳原子。因此，我们单独或混合测试了它们的两种对映体。当一起测试三种化合物时，我们强调了对寄生蜂的吸引力水平的协同效应，以及针对它们单独测试记录的反应。这些行为反应得到了受测植物释放的顶空挥发物特征的支持。这些结果为地下植物间交流的机制提供了新的线索，并促进使用生物源化学信息素来可持续地保护农作物。
In pioneering studies on plant-aphid interactions, we have observed that Vicia faba plants infested by aphids can transmit signals via the rhizosphere that induce aboveground defence in intact, neighbouring plants. The aphid parasitoid Aphidius ervi is significantly attracted towards intact broad bean plants grown in a hydroponic solution previously harbouring Acyrtosiphon pisum-infested plants. To identify the rhizosphere signal(s) possibly mediating this belowground plant-plant communication, root exudates were collected using Solid-Phase Extraction (SPE) from 10-day old A. pisum-infested and un-infested Vicia faba plants hydroponically grown. To verify the ability of these root exudates to trigger defence mechanisms against the aphids we added them to V. fabae plants grown in hydroponic solution, and tested these plants in the wind-tunnel bioassay to assess their attractiveness towards the aphids’ parasitoids A. ervi. We identified three small volatile lipophilic molecules as plant defence elicitors: 1-octen-3-ol, sulcatone and sulcatol, in SPE extracts of A. pisum-infested broad bean plants. In wind tunnel assays, we recorded a significant increase in the attractiveness towards A. ervi of V. faba plants grown in hydroponic solution treated with these compounds, compared to plants grown in hydroponic treated with ethanol (control). Both 1-octen-3-ol and sulcatol have asymmetrically substituted carbon atoms at positions 3 and 2, respectively. Hence, we tested both their enantiomers alone or in mixture. We highlighted a synergistic effect on the level of attractiveness towards the parasitoid when testing the three compounds together in respect to the response recorded against them singly tested. These behavioural responses were supported by the characterization of headspace volatiles released by tested plants. These results shed new light on the mechanisms underlying plant-plant communication belowground and prompt the use of bio-derived semiochemicals for a sustainable protection of agricultural crops.
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影响因子：
16.6
作者：
Erb, Matthias;Veyrat, Nathalie;Robert, Christelle A. M.;Xu, Hao;Frey, Monika;Ton, Jurriaan;Turlings, Ted C. J.
通讯作者：
Turlings, Ted C. J.
DOI：
10.1177/1082013221998843
发表时间：
2022-03
期刊：
Food science and technology international = Ciencia y tecnologia de los alimentos internacional
影响因子：
--
作者：
Akkad R;Kharraz E;Han J;House JD;Curtis JM
通讯作者：
Curtis JM
影响因子：
1.8
作者：
Bendera, M.;Ekesi, S.;Torto, B.
通讯作者：
Torto, B.
DOI：
10.3390/antiox11050931
发表时间：
2022-05-09
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.6
作者：
Coppola M;Cascone P;Madonna V;Di Lelio I;Esposito F;Avitabile C;Romanelli A;Guerrieri E;Vitiello A;Pennacchio F;Rao R;Corrado G
通讯作者：
Corrado G